CD81
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
1 CTGAGGTCCTGGCCGAAAC GCACCCATCACCACCACAG 782bp chr11:2398360+2399141
2 AGCAGCAGAGCTGACACCTG CCCGACTGTCTTTCACCCTC 351bp chr11:2411507+2411857
3 ATGTGAGGTCCCTTGCTGCT GAACTGGCCACGATGGAGAC 441bp chr11:2415173+2415613
4 CGCTCACTCCTGGTCAGGT TGGAGATCCTCCTGGCAAGT 357bp chr11:2416062+2416418
5 ACTTGCCAGGAGGATCTCCA TTCCCACCCGAGGACCAGTT 509bp chr11:2416399+2416907
6 CATGGGTTCCCTAGAGCCAC CCAGAGCCTCCACCTTCTTC 402bp chr11:2417029+2417430
7 CCAGGATTCCCCTCTACGCT TACACGGAGCTGTTCCGGAT 345bp chr11:2417748+2418092
8a TCCGGGAAGCTGTACCTCAT GGAGGGAACAAGGTGAGCTG 603bp chr11:2417891+2418493

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
1 CTGAGGTCCTGGCCGAAAC GCACCCATCACCACCACAG 782bp chr11:2398360+2399141
2 AGCAGCAGAGCTGACACCTG CCCGACTGTCTTTCACCCTC 351bp chr11:2411507+2411857
3 ATGTGAGGTCCCTTGCTGCT GAACTGGCCACGATGGAGAC 441bp chr11:2415173+2415613
4 CGCTCACTCCTGGTCAGGT TGGAGATCCTCCTGGCAAGT 357bp chr11:2416062+2416418
5 ACTTGCCAGGAGGATCTCCA TTCCCACCCGAGGACCAGTT 509bp chr11:2416399+2416907
6 CATGGGTTCCCTAGAGCCAC CCAGAGCCTCCACCTTCTTC 402bp chr11:2417029+2417430
7 CCAGGATTCCCCTCTACGCT TACACGGAGCTGTTCCGGAT 345bp chr11:2417748+2418092
8a TCCGGGAAGCTGTACCTCAT GGAGGGAACAAGGTGAGCTG 603bp chr11:2417891+2418493

IV. Ethanol precipitation

V. Sequencing analysis