CD79A
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TTGGGGTAGGGATCTGG TGTGAGACTGTGGACGC 363bp chr19:42381311+42381673
2 TTCTTCACTTTCCCGCATC GGTACTGGCTCTATCCCC 571bp chr19:42382874+42383444
3 CAGAGGGACGGACATTCTCC GCCCCTATATGGTGGGTGCT 386bp chr19:42383473+42383858
4a GTGGCCTTGGGGACATCTCT ATATTCATCCCCGGCATC 166bp chr19:42384619+42384784
4b ACCCCTACAGAAACGATGGCAG GAGCAGTCGTCCAGGTTCAGG 230bp chr19:42384727+42384956
5 TGGGGTAGGGGCAGTTGTGT TAAGGAAGCCCCCAGAAG 331bp chr19:42384824+42385154

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TTGGGGTAGGGATCTGG TGTGAGACTGTGGACGC 363bp chr19:42381311+42381673
2 TTCTTCACTTTCCCGCATC GGTACTGGCTCTATCCCC 571bp chr19:42382874+42383444
3 CAGAGGGACGGACATTCTCC GCCCCTATATGGTGGGTGCT 386bp chr19:42383473+42383858
4a GTGGCCTTGGGGACATCTCT ATATTCATCCCCGGCATC 166bp chr19:42384619+42384784
4b ACCCCTACAGAAACGATGGCAG GAGCAGTCGTCCAGGTTCAGG 230bp chr19:42384727+42384956
5 TGGGGTAGGGGCAGTTGTGT TAAGGAAGCCCCCAGAAG 331bp chr19:42384824+42385154

IV. Ethanol precipitation

V. Sequencing analysis