NBN
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AACCTTACATTCAGCGACACC AATATGCGCTTGCCATACAGC 455bp chr8:90996596-90997050
2 AACCTTTGATAGCCTTCAGTGAG TGACAAATCCTGTGAACTCTCTCTC 433bp chr8:90994850-90995282
3 AGCAGTAATTGTTGTCTGCCG CCATGGCACAGAGTCCAATAC 419bp chr8:90993463-90993881
4 AGGTTACAAAGCTTAATGATGAGG TGGTATACAAAGGGATGGAGTGG 482bp chr8:90992813-90993294
5 TAATGCAGTGACCAAAGACCG CCTGAAACAAGCATTAAAGAGG 384bp chr8:90990355-90990738
6 TGTCACCTGCCACCATATTTC ATCACTGGGCAGGTCTGGTG 477bp chr8:90983242-90983718
7 GAATTGTACTTCTATGTTTCCCAAATC AGGTTAAACATAAAATCTCCTACTTGC 343bp chr8:90982534-90982876
8 TCTGCATTTGAAATTCTGTCTATACC TCATAACAGTTTCTGCTACATGGC 420bp chr8:90976451-90976870
9 TTGTTGGTACCACTCATTGGAG CCTGCTGACCCTTGTCCTAAG 436bp chr8:90970795-90971230
10 TAAGGTATCATCCTTCCACAGG GCAGCAGCAGAAGCATACTTAATC 586bp chr8:90967397-90967982
11 GTGAACTAAATGGAGGGAGTGATG TGAGTGTTAAAGACATTAATGGATGC 658bp chr8:90965362-90966019
12 TGATAATCCTACCATCTACAGACAACC TCAAGGCACAATCATGAAGTAAG 609bp chr8:90959920-90960528
13 TTGATTCCAAATACAAAGTTCAGG TTATCTTTGTTTAGCATCACTGGTATC 387bp chr8:90958290-90958676
14 TGCATGATTTACCATCTTTGC AAGAATTTGCTTGAAGGCCAC 286bp chr8:90955405-90955690
15 TTGTAGAGGCAAGAACTCAGATG GGACTAGGTGTCTATGAGGACAGAAG 298bp chr8:90949144-90949441
16 ATCCTATTTGCCAAAGTGTGAC TTGTTGGCCTGAAGTAGATGC 197bp chr8:90947747-90947943

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AACCTTACATTCAGCGACACC AATATGCGCTTGCCATACAGC 455bp chr8:90996596-90997050
2 AACCTTTGATAGCCTTCAGTGAG TGACAAATCCTGTGAACTCTCTCTC 433bp chr8:90994850-90995282
3 AGCAGTAATTGTTGTCTGCCG CCATGGCACAGAGTCCAATAC 419bp chr8:90993463-90993881
4 AGGTTACAAAGCTTAATGATGAGG TGGTATACAAAGGGATGGAGTGG 482bp chr8:90992813-90993294
5 TAATGCAGTGACCAAAGACCG CCTGAAACAAGCATTAAAGAGG 384bp chr8:90990355-90990738
6 TGTCACCTGCCACCATATTTC ATCACTGGGCAGGTCTGGTG 477bp chr8:90983242-90983718
7 GAATTGTACTTCTATGTTTCCCAAATC AGGTTAAACATAAAATCTCCTACTTGC 343bp chr8:90982534-90982876
8 TCTGCATTTGAAATTCTGTCTATACC TCATAACAGTTTCTGCTACATGGC 420bp chr8:90976451-90976870
9 TTGTTGGTACCACTCATTGGAG CCTGCTGACCCTTGTCCTAAG 436bp chr8:90970795-90971230
10 TAAGGTATCATCCTTCCACAGG GCAGCAGCAGAAGCATACTTAATC 586bp chr8:90967397-90967982
11 GTGAACTAAATGGAGGGAGTGATG TGAGTGTTAAAGACATTAATGGATGC 658bp chr8:90965362-90966019
12 TGATAATCCTACCATCTACAGACAACC TCAAGGCACAATCATGAAGTAAG 609bp chr8:90959920-90960528
13 TTGATTCCAAATACAAAGTTCAGG TTATCTTTGTTTAGCATCACTGGTATC 387bp chr8:90958290-90958676
14 TGCATGATTTACCATCTTTGC AAGAATTTGCTTGAAGGCCAC 286bp chr8:90955405-90955690
15 TTGTAGAGGCAAGAACTCAGATG GGACTAGGTGTCTATGAGGACAGAAG 298bp chr8:90949144-90949441
16 ATCCTATTTGCCAAAGTGTGAC TTGTTGGCCTGAAGTAGATGC 197bp chr8:90947747-90947943

IV. Ethanol precipitation

V. Sequencing analysis