NCF2
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CCCAGAAAGTGAACACCTTGG AGAGGTTAGGTTTCCGAAATGC 376bp chr1:183559204-183559579
3 CACACCATTGTCCCAGACTTC CCTCACCTGCTGATTTATTGC 446bp chr1:183555871-183556316
4 CTGAAAGGCTTCTTGTCCCAG GCTCCTGGAGGTGTCAACAG 324bp chr1:183546636-183546959
5 AGGCTACCAGGCAACATTCTC CAGCTTCTCAGCACAGATTCAAG 409bp chr1:183543449-183543857
6 GAGGAATGACAACACTCATTTGG ACAGAGCCACAAGGAGGCTAC 353bp chr1:183542216-183542568
7 ACTGTCCACGTCTGAAACCAG ACTGAGGGCCACTTGAAGGAG 304bp chr1:183539792-183540095
8 TGACTGTGCCCTCTTCCTAGAC TCAACAAGATTTAGACCCTGACC 315bp chr1:183538167-183538481
9-10 CGGCAGTTGCAGTTAGATGTG GGTAGCTGGTGCCATCTCAAG 704bp chr1:183535905-183536608
11 TTAGGACGTTATCTGCATGTGG AGCTGAGTGACATGTTGGGAG 320bp chr1:183534716-183535035
12 GTGTTCATGACCTTCATGCTTC TGTGCATGATAGTGGAGGAGG 400bp chr1:183532960-183533359
13-14 GTGCCAAATCACGAAACTGC GCCTAGTACACAGAAGGTGCTTG 570bp chr1:183532238-183532807
15 CTGATTTACCCAACCATCCAG TCTGAGTGCTCAACTTTGCTC 522bp chr1:183529068-183529589
16a CAAATTCTGTGTGGAATAGCCAG ACCTGGAAGACTCTCTCGTGC 473bp chr1:183524974-183525446

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CCCAGAAAGTGAACACCTTGG AGAGGTTAGGTTTCCGAAATGC 376bp chr1:183559204-183559579
3 CACACCATTGTCCCAGACTTC CCTCACCTGCTGATTTATTGC 446bp chr1:183555871-183556316
4 CTGAAAGGCTTCTTGTCCCAG GCTCCTGGAGGTGTCAACAG 324bp chr1:183546636-183546959
5 AGGCTACCAGGCAACATTCTC CAGCTTCTCAGCACAGATTCAAG 409bp chr1:183543449-183543857
6 GAGGAATGACAACACTCATTTGG ACAGAGCCACAAGGAGGCTAC 353bp chr1:183542216-183542568
7 ACTGTCCACGTCTGAAACCAG ACTGAGGGCCACTTGAAGGAG 304bp chr1:183539792-183540095
8 TGACTGTGCCCTCTTCCTAGAC TCAACAAGATTTAGACCCTGACC 315bp chr1:183538167-183538481
9-10 CGGCAGTTGCAGTTAGATGTG GGTAGCTGGTGCCATCTCAAG 704bp chr1:183535905-183536608
11 TTAGGACGTTATCTGCATGTGG AGCTGAGTGACATGTTGGGAG 320bp chr1:183534716-183535035
12 GTGTTCATGACCTTCATGCTTC TGTGCATGATAGTGGAGGAGG 400bp chr1:183532960-183533359
13-14 GTGCCAAATCACGAAACTGC GCCTAGTACACAGAAGGTGCTTG 570bp chr1:183532238-183532807
15 CTGATTTACCCAACCATCCAG TCTGAGTGCTCAACTTTGCTC 522bp chr1:183529068-183529589
16a CAAATTCTGTGTGGAATAGCCAG ACCTGGAAGACTCTCTCGTGC 473bp chr1:183524974-183525446

IV. Ethanol precipitation

V. Sequencing analysis