NFKBIA
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TGTCCGTGACCCTAGTGGCT AAACTCCCGCCCCGCCTTAT 868bp chr14:35873292-35874159
2 GCCAGGAACACTCAGCTCATAA AATAGCAGAGGCTCCAGGTG 468bp chr14:35872692-35873159
3 CCTGTCTAGGAGGAGCAGCA TGCCTGGACTCCTTAAGTTGG 406bp chr14:35872267-35872672
4-5 CCAACTTAAGGAGTCCAGGCA TGGACTCCATTCTCCATAGCC 929bp chr14:35871359-35872287
6a GCTATGGAGAATGGAGTCCAAGA GGTCAGTCACTCGAAGCACAA 483bp chr14:35870896-35871378

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AAATCCCCAGCCAGCGTTTA AAACTCCCGCCCCGCCTTAT 713bp chr14:35873292-35874004
2 GCCAGGAACACTCAGCTCATAA AATAGCAGAGGCTCCAGGTG 468bp chr14:35872692-35873159
3 CCTGTCTAGGAGGAGCAGCA TGCCTGGACTCCTTAAGTTGG 406bp chr14:35872267-35872672
4-5 CCAACTTAAGGAGTCCAGGCA TGGACTCCATTCTCCATAGCC 929bp chr14:35871359-35872287
6a GCTATGGAGAATGGAGTCCAAGA GAAAAATAAAACTTTTTTTTTGTAC 222bp chr14:35871157-35871378

IV. Ethanol precipitation

V. Sequencing analysis