NHEJ1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 GGAGGTAGTAAAGTCAGTGCTC GTCTCAACCCGATTCAACCT 450bp chr2:220022812-220023261
3 ATGTCCATTGCCTTCGTGTT TTTTTGCCTTCTGTTTAGTCA 461bp chr2:220022050-220022510
4 AATGGGTTACTTGCCTATGA CATGGTGGCGTGCGTCTACA 589bp chr2:220012064-220012652
5 TCCCACTGAACTAGGTGTCTC GGAAGTAGGGCAAAGACACA 239bp chr2:220011359-220011597
6 TGGGGTATTAGATCGCTTCT GGACAGCATAAACCTAGATCC 248bp chr2:219942747-219942994
7 AGCTGGCTGTTTTGAGTCGA GCCAACTCTCAGAACACCAT 404bp chr2:219941925-219942328
8a CCTAACCAGGAGCCATGAGAG CATCAACTTCAGTTCTCTCGCC 313bp chr2:219940959-219941271

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 GGAGGTAGTAAAGTCAGTGCTC GTCTCAACCCGATTCAACCT 450bp chr2:220022812-220023261
3 ATGTCCATTGCCTTCGTGTT TTTTTGCCTTCTGTTTAGTCA 461bp chr2:220022050-220022510
4 AATGGGTTACTTGCCTATGA CATGGTGGCGTGCGTCTACA 589bp chr2:220012064-220012652
5 TCCCACTGAACTAGGTGTCTC GGAAGTAGGGCAAAGACACA 239bp chr2:220011359-220011597
6 TGGGGTATTAGATCGCTTCT GGACAGCATAAACCTAGATCC 248bp chr2:219942747-219942994
7 AGCTGGCTGTTTTGAGTCGA GCCAACTCTCAGAACACCAT 404bp chr2:219941925-219942328
8a CCTAACCAGGAGCCATGAGAG CATCAACTTCAGTTCTCTCGCC 313bp chr2:219940959-219941271

IV. Ethanol precipitation

V. Sequencing analysis