PRF1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 GGAAGGGAGCAGTCATCCTC GGCTCCTAGACCACCCAGAG 680bp chr10:72360054-72360733
3a GCCCACTTACATGTGACCTTG ACACACACTGGCATGGGTC 749bp chr10:72358288-72359036
3b GGTGGACTACACCCTGGAAC GCCGCATTCAAAGCCATCCT 797bp chr10:72357667-72358463

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 GGAAGGGAGCAGTCATCCTC GGCTCCTAGACCACCCAGAG 680bp chr10:72360054-72360733
3a GCCCACTTACATGTGACCTTG ACACACACTGGCATGGGTC 749bp chr10:72358288-72359036
3b GGTGGACTACACCCTGGAAC GCCGCATTCAAAGCCATCCT 797bp chr10:72357667-72358463

IV. Ethanol precipitation

V. Sequencing analysis