SH2D1A
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1b TTTGCACATCTGGCTGAACT AGGCGAAAGTGTGTTCCACCA 429bp chrX:123480378+123480806
2 GTGTCCTAGTATATGTGACATT GGCTAAACAGGACTGGGACCA 356bp chrX:123499508+123499863
3 CATTAGCTCCTCTTGCAGGGA CCTCTCATTTGACTTGCTGGCT 485bp chrX:123503827+123504311
4 TGTGTCATTGTGAGTTTTATGCAG AAAGTCCATTTCAGCTTTGACG 313bp chrX:123505092+123505404

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1b TTTGCACATCTGGCTGAACT AGGCGAAAGTGTGTTCCACCA 429bp chrX:123480378+123480806
2 GTGTCCTAGTATATGTGACATT GGCTAAACAGGACTGGGACCA 356bp chrX:123499508+123499863
3 CATTAGCTCCTCTTGCAGGGA CCTCTCATTTGACTTGCTGGCT 485bp chrX:123503827+123504311
4 TGTGTCATTGTGAGTTTTATGCAG AAAGTCCATTTCAGCTTTGACG 313bp chrX:123505092+123505404

IV. Ethanol precipitation

V. Sequencing analysis