ORAI1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ACAACAACGCCCACTTCTTG CACGGTTTCTCCCAGCTCTTC 771bp chr12:122064310+122065080
2a GTAGGGCTTTCTGCCACTCTC GTCCTGTAAGCGGGCAAACTC 741bp chr12:122078755+122079495
2b GTCTTCGCCGTCCACTTCTAC GCCTCCTGAATAGCTGAGACC 680bp chr12:122079397+122080076

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ACAACAACGCCCACTTCTTG CACGGTTTCTCCCAGCTCTTC 771bp chr12:122064310+122065080
2a GTAGGGCTTTCTGCCACTCTC GTCCTGTAAGCGGGCAAACTC 741bp chr12:122078755+122079495
2b GTCTTCGCCGTCCACTTCTAC GCCTCCTGAATAGCTGAGACC 680bp chr12:122079397+122080076

IV. Ethanol precipitation

V. Sequencing analysis