TYK2
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 GGTGGGTGGAAGGTTGAAG GTGGGTGGGTGGATAGACG 377bp chr19:10488802-10489178
4-5 AGAGGGAGTGTGAAGGCAGAC CCAGATAGCATGAGTTGAAACC 576bp chr19:10478615-10479190
6 CCTCTGGGCTAGAGAGGAACG ATGCAAAGTCTACCCTGGCTC 306bp chr19:10477013-10477318
7 CCACTTGCCCTGTGTCACC GGATGAACACAGAAACCCACC 522bp chr19:10476127-10476648
8-9 AGGAGGTATAAACGGGCATTG TAGGGCTCACAGTCTAGTTGGC 557bp chr19:10475226-10475782
10 CTTGTCCTAGCTGTGTGTGTGC GCAGAGGTGGGAGCAGTAAG 300bp chr19:10473146-10473445
11-12 CACGTTCCCTGCCTGGTC TTAGCAGCTGATCTCCCAGG 517bp chr19:10472672-10473188
13-14 GACCAGAAGGAGATCACCCAG TCAGGGAGTGGGTATAAGTCAGG 654bp chr19:10472121-10472774
15 AACCCAGACCAAACTCCACC ACAGGGCGAAACTCCACC 403bp chr19:10469719-10470121
16-17 TTGGCGTCTGTGCCTCTC GTCCTCTGGATTTCTTGGAGG 491bp chr19:10468377-10468867
18 AACTGATAAGAGCGGGCACC CACTGCAAGAAATTGGCACAC 390bp chr19:10467119-10467508
19 CTGTGGCTGGCTTTGTGACTC CTAGGCCTCCCTCAAAGCAG 312bp chr19:10465058-10465369
20 AGGATCTGCCTGGAGGAGTG CACACCCACGCTCTAACCAC 347bp chr19:10464659-10465005
21 CCCACCTGAGAACTGGGTC GTGAGAGCAGACTGCACCG 346bp chr19:10464116-10464461
22 CCTCCTGGCTGCTCAGGTC ACCCTGCCTGTTCCAAGTG 311bp chr19:10463545-10463855
23 AGTCTCAGGTGAGGGCTTCAG TCTCTCCGTCCTGTCCTGTC 566bp chr19:10462746-10463311
24 TTGGAACCCATGCAGTCG CAGAATGGGTATGAGGTTCTCG 653bp chr19:10461567-10462219
25 CCACTATCCTGCTGTGGAGAG AGACCAGACTCCCAGCTAACC 552bp chr19:10461148-10461699

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 GGTGGGTGGAAGGTTGAAG GTGGGTGGGTGGATAGACG 377bp chr19:10488802-10489178
4-5 AGAGGGAGTGTGAAGGCAGAC CCAGATAGCATGAGTTGAAACC 576bp chr19:10478615-10479190
6 CCTCTGGGCTAGAGAGGAACG ATGCAAAGTCTACCCTGGCTC 306bp chr19:10477013-10477318
7 CCACTTGCCCTGTGTCACC GGATGAACACAGAAACCCACC 522bp chr19:10476127-10476648
8-9 AGGAGGTATAAACGGGCATTG TAGGGCTCACAGTCTAGTTGGC 557bp chr19:10475226-10475782
10 CTTGTCCTAGCTGTGTGTGTGC GCAGAGGTGGGAGCAGTAAG 300bp chr19:10473146-10473445
11-12 CACGTTCCCTGCCTGGTC TTAGCAGCTGATCTCCCAGG 517bp chr19:10472672-10473188
13-14 GACCAGAAGGAGATCACCCAG TCAGGGAGTGGGTATAAGTCAGG 654bp chr19:10472121-10472774
15 AACCCAGACCAAACTCCACC CAGGCTGGTCTCGAACTC chr19:10470102-?
16-17 TTGGCGTCTGTGCCTCTC GTCCTCTGGATTTCTTGGAGG 491bp chr19:10468377-10468867
18 TCCACTCTGGGGACTTGACT CACTGCAAGAAATTGGCACAC 364bp chr19:10467119-10467482
19 CTGTGGCTGGCTTTGTGACTC CTAGGCCTCCCTCAAAGCAG 312bp chr19:10465058-10465369
20 AGGATCTGCCTGGAGGAGTG CACACCCACGCTCTAACCAC 347bp chr19:10464659-10465005
21 CCCACCTGAGAACTGGGTC GTGAGAGCAGACTGCACCG 346bp chr19:10464116-10464461
22 CCTCCTGGCTGCTCAGGTC ACCCTGCCTGTTCCAAGTG 311bp chr19:10463545-10463855
23 AGTCTCAGGTGAGGGCTTCAG TCTCTCCGTCCTGTCCTGTC 566bp chr19:10462746-10463311
24 TTGGAACCCATGCAGTCG CAGAATGGGTATGAGGTTCTCG 653bp chr19:10461567-10462219
25 CCACTATCCTGCTGTGGAGAG AGACCAGACTCCCAGCTAACC 552bp chr19:10461148-10461699

IV. Ethanol precipitation

V. Sequencing analysis