WAS
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AAGCTCAGCCTAACGAGGAG GGCAAGTAGACATTGAGATCTTCC 473bp chrX:48542073+48542545
2 TCCAAGACCTTGTGGCTACC TGAGGTCTTGAAGCTATGGACAC 293bp chrX:48542613+48542905
3-4-5 ACACCAACTCTCTGGTGCTG GAAGAATCAGCACGCACCTC 755bp chrX:48543786+48544540
6 CACCACCAACACCAGCCAATG ACACATCCATGCACCTGCTCG 313bp chrX:48544353+48544665
7 TTCCCTCAAGGCTTCCGTTTC TGCCCATCCTTCCATTCACTC 351bp chrX:48545084+48545434
8-9 AGAGGGCAAGAGGGTTTCACTATG AGGTGCTCCCATAAGGACTGAG 571bp chrX:48546364+48546934
10 ACGCTCCCTTCTCTAGCCCAAGCAG TCAAGGCTTCCCACCAACCTTTCAAC 745bp chrX:48546861+48547605
11 AGGAGAGTGCCAGGCCTTAG AACGAGGCTGACACAAGATTC 419bp chrX:48547545+48547963
12 AGTCCTCACCTCCCAGGCCCTATG CCCTGACTGCTCCATCTGAATGTTG 795bp chrX:48549424+48550218

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AAGCTCAGCCTAACGAGGAG GGCAAGTAGACATTGAGATCTTCC 473bp chrX:48542073+48542545
2 TCCAAGACCTTGTGGCTACC TGAGGTCTTGAAGCTATGGACAC 293bp chrX:48542613+48542905
3-4-5 ACACCAACTCTCTGGTGCTG GAAGAATCAGCACGCACCTC 755bp chrX:48543786+48544540
6 CACCACCAACACCAGCCAATG ACACATCCATGCACCTGCTCG 313bp chrX:48544353+48544665
7 TTCCCTCAAGGCTTCCGTTTC TGCCCATCCTTCCATTCACTC 351bp chrX:48545084+48545434
8-9 AGAGGGCAAGAGGGTTTCACTATG AGGTGCTCCCATAAGGACTGAG 571bp chrX:48546364+48546934
10 ACGCTCCCTTCTCTAGCCCAAGCAG TCAAGGCTTCCCACCAACCTTTCAAC 745bp chrX:48546861+48547605
11 AGGAGAGTGCCAGGCCTTAG AACGAGGCTGACACAAGATTC 419bp chrX:48547545+48547963
12 AGTCCTCACCTCCCAGGCCCTATG CCCTGACTGCTCCATCTGAATGTTG 795bp chrX:48549424+48550218

IV. Ethanol precipitation

V. Sequencing analysis