LPIN2
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 AAGGCTAATGATATGAAATCTGGC ATAGCGATTTATTCATAGAGGATGAC 428bp chr18:2960543-2960970
3 GGGCAAGTAGCAGTGTTTCTC CTTGCCAACAACAGTCCTCTG 361bp chr18:2954383-2954743
4 TGCTTTAATTCACTGTCGAGTTTG CTGGCTTCACATGAACTCTAGG 420bp chr18:2950994-2951413
5 GATTGTTTGTAGTATCAGTAGGTTTGG TTGGCTGTGTGAGAGAAATGG 307bp chr18:2940494-2940800
6 TCAGGCTCAGCAAGACTGAAG GGCAGAGGAATTCGTCACTTG 255bp chr18:2939413-2939667
7 TGAACACATTGCCAGCTTAGG AGCCATCACGTTATGTGGAAC 509bp chr18:2937609-2938117
8 ATGGCTGGGAACCAACCTAAG CTGAAGCCATCATCAGATTTAGC 394bp chr18:2934220-2934613
9 TGATTGTAGGTCCATCCGTTC TATCCTGAAATGGCTACACGG 406bp chr18:2931165-2931570
10 TAGCCTTCAATTTCTGCAATG CCACTGGCTAATTTATATGAGATGAC 314bp chr18:2928936-2929249
11 GAAGTTGTGCTTGAAGCGAAG TTGTGTAAGTACTAGACACTGCGG 310bp chr18:2928527-2928836
12 AGAAGGTAGGTGCTGTGTGGC ACAACACCTGCACGTCCTAAG 310bp chr18:2927561-2927870
13 TGCTGATGAACTTGGCTTATCTG CAACTTAGAAGTAATGGCCCTGC 219bp chr18:2926652-2926870
14 GCGTACAGAGCTGTGGATATGG CTGTGGATAGGCATTGACACG 440bp chr18:2925085-2925524
15 GGATTCCGAGACACCAGTTG GTTCGGAGGTAACTGACTGCC 561bp chr18:2924076-2924636
16 ATTGGCAGCTGATAGTCAACC TCATGTGGACTGAAGACTTACACC 264bp chr18:2923681-2923944
17 TCCCTTAGCCAGAACATGTGG CCAACATCTGACTTCTGTTCCC 298bp chr18:2921946-2922243
18 CCTACCACATACTGCTCACTTGC ATCTGCACCTGCAGGACCTC 564bp chr18:2921341-2921904
19 TGCTTCTGGCTCACAAGGAG CCAACTGTACCCCTGGCTG 240bp chr18:2920722-2920961
20a TGTGCCTTTGACCGATCTTG CCTGGTTACAGAAGCCACCTC 607bp chr18:2919888-2920494

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 AAGGCTAATGATATGAAATCTGGC ATAGCGATTTATTCATAGAGGATGAC 428bp chr18:2960543-2960970
3 GGGCAAGTAGCAGTGTTTCTC CTTGCCAACAACAGTCCTCTG 361bp chr18:2954383-2954743
4 TGCTTTAATTCACTGTCGAGTTTG CTGGCTTCACATGAACTCTAGG 420bp chr18:2950994-2951413
5 GATTGTTTGTAGTATCAGTAGGTTTGG TTGGCTGTGTGAGAGAAATGG 307bp chr18:2940494-2940800
6 TCAGGCTCAGCAAGACTGAAG GGCAGAGGAATTCGTCACTTG 255bp chr18:2939413-2939667
7 TGAACACATTGCCAGCTTAGG AGCCATCACGTTATGTGGAAC 509bp chr18:2937609-2938117
8 ATGGCTGGGAACCAACCTAAG CTGAAGCCATCATCAGATTTAGC 394bp chr18:2934220-2934613
9 TGATTGTAGGTCCATCCGTTC TATCCTGAAATGGCTACACGG 406bp chr18:2931165-2931570
10 TAGCCTTCAATTTCTGCAATG CCACTGGCTAATTTATATGAGATGAC 314bp chr18:2928936-2929249
11 GAAGTTGTGCTTGAAGCGAAG TTGTGTAAGTACTAGACACTGCGG 310bp chr18:2928527-2928836
12 AGAAGGTAGGTGCTGTGTGGC ACAACACCTGCACGTCCTAAG 310bp chr18:2927561-2927870
13 TGCTGATGAACTTGGCTTATCTG CAACTTAGAAGTAATGGCCCTGC 219bp chr18:2926652-2926870
14 GCGTACAGAGCTGTGGATATGG CTGTGGATAGGCATTGACACG 440bp chr18:2925085-2925524
15 GGATTCCGAGACACCAGTTG GTTCGGAGGTAACTGACTGCC 561bp chr18:2924076-2924636
16 ATTGGCAGCTGATAGTCAACC TCATGTGGACTGAAGACTTACACC 264bp chr18:2923681-2923944
17 TCCCTTAGCCAGAACATGTGG CCAACATCTGACTTCTGTTCCC 298bp chr18:2921946-2922243
18 CCTACCACATACTGCTCACTTGC ATCTGCACCTGCAGGACCTC 564bp chr18:2921341-2921904
19 TGCTTCTGGCTCACAAGGAG CCAACTGTACCCCTGGCTG 240bp chr18:2920722-2920961
20a TGTGCCTTTGACCGATCTTG CCTGGTTACAGAAGCCACCTC 607bp chr18:2919888-2920494

IV. Ethanol precipitation

V. Sequencing analysis