LCK
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 AGTCACTGGAGGGTTTTCTG CTGTATTTCAGGGGAGAGGG 837bp chr1:32739320+32740156
3-4 AGGCTGAGAGCAGAGCAGG TCAGCAATACTGAGCCTCG 569bp chr1:32740251+32740819
5-6 AATTCGAGGCTCAGTATTGC AAGAACGCAGTGAGTCTGGC 686bp chr1:32740797+32741482
7 GGCCAGACTCACTGCGTTC TCAGGTTCCACACCTCACC 330bp chr1:32741462+32741791
8-9 CAACGGTGGCTTCTACATCTC CAGCTCACTGCAACTGTAGCC 1173bp chr1:32741594+32742766
10-11 CTGAACACACACTCCTCCATTC TTAATGGCTTCTGGCGCTG 594bp chr1:32745129+32745722
12 CTGCCAACATTCTGGTGTCTG GATACGGAGATGGAAGCCAG 497bp chr1:32745500+32745996
13a CTTCCAGTGCCTGACCTTG TGTCCAAGACATACATGCACAG 473bp chr1:32751070+32751542

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 AGTCACTGGAGGGTTTTCTG CTGTATTTCAGGGGAGAGGG 837bp chr1:32739320+32740156
3-4 AGGCTGAGAGCAGAGCAGG TCAGCAATACTGAGCCTCG 569bp chr1:32740251+32740819
5-6 AATTCGAGGCTCAGTATTGC AAGAACGCAGTGAGTCTGGC 686bp chr1:32740797+32741482
7 GGCCAGACTCACTGCGTTC TCAGGTTCCACACCTCACC 330bp chr1:32741462+32741791
8-9 CAACGGTGGCTTCTACATCTC CAGCTCACTGCAACTGTAGCC 1173bp chr1:32741594+32742766
10-11 CTGAACACACACTCCTCCATTC TTAATGGCTTCTGGCGCTG 594bp chr1:32745129+32745722
12 CTGCCAACATTCTGGTGTCTG GATACGGAGATGGAAGCCAG 497bp chr1:32745500+32745996
13a CTTCCAGTGCCTGACCTTG TGTCCAAGACATACATGCACAG 473bp chr1:32751070+32751542

IV. Ethanol precipitation

V. Sequencing analysis