XIAP
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2a GAATGTTTCTTAGCGGTCGTG TTCCTCGGGTATATGGTGTCTG 628bp chrX:123019355+123019982
2b TGCCACGCAGTCTACAAATTC GGGGCTAAGGGCATCTATTTAC 655bp chrX:123019815+123020469
3 TTGCAGTGAACCAAGACTGC GAATTTCAAGCCATAGTGAAGGC 555bp chrX:123022122+123022676
4 GACAGTGGGATAGGGAATTGG CTGCCCAGCTAGCTCTCATC 471bp chrX:123024926+123025396
5 TCAAATGGAAATGATTGCCTC GGTGACCTTGGAAGAACATGC 522bp chrX:123026517+123027038
6 AGCTTTGCTTTGCTGGTTTG TCCATATCCACCTGTCCCAG 440bp chrX:123034202+123034641
7a CGGGTGAGTCATCCTCATATC AAATCCAGATGATTAATTCACTTACAC 655bp chrX:123040705+123041359

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2a GAATGTTTCTTAGCGGTCGTG TTCCTCGGGTATATGGTGTCTG 628bp chrX:123019355+123019982
2b TGCCACGCAGTCTACAAATTC GGGGCTAAGGGCATCTATTTAC 655bp chrX:123019815+123020469
3 TTGCAGTGAACCAAGACTGC AATTCAAGTCTCCCACTGCA 510bp chrX:123022122+123022631
4 TGGCTCCTTAGAAGTACTGA CTGCCCAGCTAGCTCTCATC 434bp chrX:123024963+123025396
5 TCAAATGGAAATGATTGCCTC GGTGACCTTGGAAGAACATGC 522bp chrX:123026517+123027038
6 AGCTTTGCTTTGCTGGTTTG TCCATATCCACCTGTCCCAG 440bp chrX:123034202+123034641
7a CGGGTGAGTCATCCTCATATC AAATCCAGATGATTAATTCACTTACAC 655bp chrX:123040705+123041359

IV. Ethanol precipitation

V. Sequencing analysis