MYD88
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TAGCACCATCACCAGACCCT GTTCTCCCTCTGCAGGCTTCT 744bp chr3:38179910+38180653
2 ACTTCTCAGAGCCGTTGAGCTT CACAGCAATCCAGAGGTAGGA 468bp chr3:38181197+38181664
03-04 ATCATCTTGGGAAGGGTGCAG AGGGAAGGCTGACAATCCAG 648bp chr3:38181765+38182412
05a TGAATGTGTGCCAGGGGTACT CCCAGAGCTATGCTTCACCA 672bp chr3:38182502+38183173

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TAGCACCATCACCAGACCCT GTTCTCCCTCTGCAGGCTTCT 744bp chr3:38179910+38180653
2 ACTTCTCAGAGCCGTTGAGCTT CACAGCAATCCAGAGGTAGGA 468bp chr3:38181197+38181664
03-04 CCAGGGTTGCCTAGGCA AGGGAAGGCTGACAATCCAG 624bp chr3:38181789+38182412
05a TGAATGTGTGCCAGGGGTACT CCCAGAGCTATGCTTCACCA 672bp chr3:38182502+38183173

IV. Ethanol precipitation

V. Sequencing analysis