AK2
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGTAAGGTGAAGAGTCCGTCG CCAGGGCTTCTAGATCTCCG 341bp chr1:33502259-33502599
2 CCCATTACAGGTAAGGAAGTAGGC GCTGGTAAATACTTGAAATATGGC 423bp chr1:33489883-33490305
3-4 AGTTGGGCTAATGTCAGTCACC ACCACTTCAGCGGATGTCAC 547bp chr1:33486826-33487372
5 CCTGGAAGCCCAGTTATTTAGC TGACACAGGCCATGTGATTTAC 388bp chr1:33479963-33480350
6 CATCTCAAATCTGCATCTGGC CAGCTGGTAAAGCAACCTAGC 561bp chr1:33478524-33479084
7a ATGCCAGCCTCCTCTGATATG TGCTTGATTTGTTGAGCCAC 624bp chr1:33475885-33476508

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGTAAGGTGAAGAGTCCGTCG CCAGGGCTTCTAGATCTCCG 341bp chr1:33502259-33502599
2 CCCATTACAGGTAAGGAAGTAGGC GCTGGTAAATACTTGAAATATGGC 423bp chr1:33489883-33490305
3-4 AGTTGGGCTAATGTCAGTCACC ACCACTTCAGCGGATGTCAC 547bp chr1:33486826-33487372
5 CCTGGAAGCCCAGTTATTTAGC TGACACAGGCCATGTGATTTAC 388bp chr1:33479963-33480350
6 CATCTCAAATCTGCATCTGGC CAGCTGGTAAAGCAACCTAGC 561bp chr1:33478524-33479084
7a ATGCCAGCCTCCTCTGATATG TGCTTGATTTGTTGAGCCAC 624bp chr1:33475885-33476508

IV. Ethanol precipitation

V. Sequencing analysis