TNFRSF13C
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CGAGCTCGGTCCCACCCAGCAG TGCCCACAGGGTCCTTTCAGCCCT 391bp chr22:42322519-42322909
2 CTTCGACCTGCTGGTCCGCCACT CAGCCTGAGCTCCTCAGCGCAAAC 761bp chr22:42321946-42322706
3a GGAGTCTTCTAGGGGAGGGAG TTAGCCTGGTCCCAGAAAGAG 373bp chr22:42321253-42321625

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CGAGCTCGGTCCCACCCAGCAG TGCCCACAGGGTCCTTTCAGCCCT 391bp chr22:42322519-42322909
2 CTTCGACCTGCTGGTCCGCCACT CCCTTAAAGCCCTTCTCTCC 713bp chr22:42321994-42322706
3a GGAGGGAGGGAAGGCTGT GAGGGCATGTGCATGCTGGA 342bp chr22:42321271-42321612

IV. Ethanol precipitation

V. Sequencing analysis