NLRP12
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCGAACTTCCTCTTCTCCACC GACAGAGCCTCACTATGTTGTCC 690bp chr19:54326990-54327679
2 GTGACCAGGGAGTTATGCAGG CCACGAATTCCTCAATCACG 285bp chr19:54318062-54318346
3a CAGGCACACACCTTCTTTGAG GAACTCGGATGAGCTCCTGG 589bp chr19:54314057-54314645
3b CAACTGCAGGGAGATGAACC CTGGACGTCTGTCTCAACAGC 537bp chr19:54313627-54314163
3c GGCCAAGTCTTCAATTACGTG CTCCTGGTCTCCTCGTTCAG 566bp chr19:54313195-54313760
3d TTTCCAGGAATTCTTTGCAGC AACACACGAACCTAAGCAGCC 572bp chr19:54312794-54313365
4 CATGCAAAGGCCATAGTTGTC ACACCATCCCGTGATAGAACC 327bp chr19:54310659-54310985
5 GAAAATAATCCACAAAACTGGAGC GCCAGCCCTGATTTAAAGGTG 345bp chr19:54308425-54308769
6 AACAGGCTGGAGCATGAGTG CCATCATTCAATATGAAGAGATTTG 306bp chr19:54307146-54307451
7 TAGTCCCAGCGATTCAGGAG TTAGGAGCAGAGACAACCTGG 461bp chr19:54304386-54304846
8 CTGTCCCTCTGTCTGCAACC GAGAAGGCGCTTCCACTGTC 336bp chr19:54301436-54301771
9 TGGGAGACAGGGTGAGACAC ATCTACCCTCATGCTCCCAGC 318bp chr19:54299047-54299364
10 GGAATTATCTTACCACGGTAGTGC TCTCAATCTGCATGAGTCTGTC 306bp chr19:54297184-54297489

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCGAACTTCCTCTTCTCCACC GACAGAGCCTCACTATGTTGTCC 690bp chr19:54326990-54327679
2 GTGACCAGGGAGTTATGCAGG CCACGAATTCCTCAATCACG 285bp chr19:54318062-54318346
3a CAGGCACACACCTTCTTTGAG GAACTCGGATGAGCTCCTGG 589bp chr19:54314057-54314645
3b CAACTGCAGGGAGATGAACC CTGGACGTCTGTCTCAACAGC 537bp chr19:54313627-54314163
3c GGCCAAGTCTTCAATTACGTG CTCCTGGTCTCCTCGTTCAG 566bp chr19:54313195-54313760
3d TTTCCAGGAATTCTTTGCAGC AACACACGAACCTAAGCAGCC 572bp chr19:54312794-54313365
4 CATGCAAAGGCCATAGTTGTC ACACCATCCCGTGATAGAACC 327bp chr19:54310659-54310985
5 GAAAATAATCCACAAAACTGGAGC GCCAGCCCTGATTTAAAGGTG 345bp chr19:54308425-54308769
6 AACAGGCTGGAGCATGAGTG CCATCATTCAATATGAAGAGATTTG 306bp chr19:54307146-54307451
7 AAGCCTCCATAATGCTTGTTG TTAGGAGCAGAGACAACCTGG 304bp chr19:54304386-54304689
8 CTGTCCCTCTGTCTGCAACC GAGAAGGCGCTTCCACTGTC 336bp chr19:54301436-54301771
9 TGGGAGACAGGGTGAGACAC ATCTACCCTCATGCTCCCAGC 318bp chr19:54299047-54299364
10 GGAATTATCTTACCACGGTAGTGC TCTCAATCTGCATGAGTCTGTC 306bp chr19:54297184-54297489

IV. Ethanol precipitation

V. Sequencing analysis