WIPF1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CCTCCATTCTCTCCAAGCAC AGCACATGTCCCTGCGCAGAT 576bp chr2:175449975-175450550
3 CACCAAGAACCTTAACAACAAGC ACTGGCTTCTTGCAGCATTAG 440bp chr2:175445839-175446278
4 GGCCAGCTCCCTGTTGTACT GAGGCTATGACCAGCCTCCA 339bp chr2:175439856-175440194
5a GCTCATAGGCTGTAGAAGTTGG CTGGAGGCTTGTTGTTCTGAG 743bp chr2:175436653-175437395
5b GGAGGAGGCTCAATACGTCAGT ATCACACGTGTCGTGTGCCACT 586bp chr2:175436257-175436842
6 GGCATGTGTCCAGCAAGTCCT CATGGTGGGCCTTAGAATGGT 472bp chr2:175432431-175432902
7 TGACCTTTGTAGCACTTCCAGCT CCTCACTGCCTGTGGAAGG 430bp chr2:175431641-175432070
8 CCACAACATACGCCAGTTAGC ACCTTTCCTCCTGCCCATACA 310bp chr2:175427159-175427468

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CCTCCATTCTCTCCAAGCAC AGCACATGTCCCTGCGCAGAT 576bp chr2:175449975-175450550
3 CACCAAGAACCTTAACAACAAGC ACTGGCTTCTTGCAGCATTAG 440bp chr2:175445839-175446278
4 GGCCAGCTCCCTGTTGTACT GAGGCTATGACCAGCCTCCA 339bp chr2:175439856-175440194
5a AGCCCTCTGAGGAAGGGACT GGAACCGCTTCCCTGTGGAT 595bp chr2:175436685-175437279
5b GGAGGAGGCTCAATACGTCAGT ATCACACGTGTCGTGTGCCACT 586bp chr2:175436257-175436842
6 GGCATGTGTCCAGCAAGTCCT GCAATGGAGTGTGATAATGATG 434bp chr2:175432469-175432902
7 TGACCTTTGTAGCACTTCCAGCT CCTCACTGCCTGTGGAAGG 430bp chr2:175431641-175432070
8 CCACAACATACGCCAGTTAGC ACCTTTCCTCCTGCCCATACA 310bp chr2:175427159-175427468

IV. Ethanol precipitation

V. Sequencing analysis