NOD2
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGTGAGGGTCATGGTCTCCAG CCAGAAACAGAGTCAGCACTCC 426bp chr16:50730916+50731341
2 CTAGAACCATGGCCAACTCG TTCCTGCCAAGAGTCAAGAGC 745bp chr16:50733286+50734030
3 ACTCATGTGGTTGTTCGGTTG GGAAATTGAGTCATAGGGAGGC 376bp chr16:50741623+50741998
4a CCTTCAGTTATGTCAGCGTCC AAGGTTGAAGAGCAGGGTCTG 684bp chr16:50744369+50745052
4b GGCCAAACCACTCTCTGTGC GACCTTGGGAAGCTGAGTCTG 587bp chr16:50744839+50745425
4c CCTGTCTTCTCATGGATGGTG CTCAGATGTCTGGCACTCAGC 636bp chr16:50745278+50745913
4d AGACACCTCTTCAATTGTGGC GCCCAGACTTCTAGAGCCCAG 675bp chr16:50745701+50746375
5 AGCACAGATGCTGGCACTTC TTGGACAGTGAGCACCAAATC 373bp chr16:50750387+50750759
6 GAGCAATTGCAGAAGTTAGCG CAGGCCAAGACCAGATCAGAC 402bp chr16:50750562+50750963
7 TCTTCAATGCTTTCTTCCTGTG CCAAGTATGAAGGTGGCAGTC 356bp chr16:50753734+50754089
8 TCTGGGATTGAGTGGTCCTG CTCCATTGCCTAACATTGTGG 327bp chr16:50756409+50756735
9 AAGAGCACCGCAATCAATTAG GTCTGTCCACACAACCGCTC 313bp chr16:50757075+50757387
10 GGCATGTGAGTTCATCATCTTC TCCACCTAGACCAAGGCAAG 294bp chr16:50759331+50759624
11 GACTGGCTAACTCCTGCAGTC AATTCTGCCATTCCTCTCTCC 266bp chr16:50763638+50763903
12a TTCCCTGCAAGAAACACTGC GATGGATGAGTCGAGACATGG 685bp chr16:50765520+50766204

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGTGAGGGTCATGGTCTCCAG CCAGAAACAGAGTCAGCACTCC 426bp chr16:50730916+50731341
2 CTAGAACCATGGCCAACTCG TTCCTGCCAAGAGTCAAGAGC 745bp chr16:50733286+50734030
3 ACTCATGTGGTTGTTCGGTTG GGAAATTGAGTCATAGGGAGGC 376bp chr16:50741623+50741998
4a CCTTCAGTTATGTCAGCGTCC AAGGTTGAAGAGCAGGGTCTG 684bp chr16:50744369+50745052
4b GGCCAAACCACTCTCTGTGC GACCTTGGGAAGCTGAGTCTG 587bp chr16:50744839+50745425
4c CCTGTCTTCTCATGGATGGTG CTCAGATGTCTGGCACTCAGC 636bp chr16:50745278+50745913
4d AGACACCTCTTCAATTGTGGC GCCCAGACTTCTAGAGCCCAG 675bp chr16:50745701+50746375
5 AGCACAGATGCTGGCACTTC TTGGACAGTGAGCACCAAATC 373bp chr16:50750387+50750759
6 GAGCAATTGCAGAAGTTAGCG CAGGCCAAGACCAGATCAGAC 402bp chr16:50750562+50750963
7 TCTTCAATGCTTTCTTCCTGTG CCAAGTATGAAGGTGGCAGTC 356bp chr16:50753734+50754089
8 TCTGGGATTGAGTGGTCCTG CTCCATTGCCTAACATTGTGG 327bp chr16:50756409+50756735
9 AAGAGCACCGCAATCAATTAG GTCTGTCCACACAACCGCTC 313bp chr16:50757075+50757387
10 GGCATGTGAGTTCATCATCTTC TCCACCTAGACCAAGGCAAG 294bp chr16:50759331+50759624
11 GACTGGCTAACTCCTGCAGTC AATTCTGCCATTCCTCTCTCC 266bp chr16:50763638+50763903
12a TTCCCTGCAAGAAACACTGC GATGGATGAGTCGAGACATGG 685bp chr16:50765520+50766204

IV. Ethanol precipitation

V. Sequencing analysis