CD3E
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1-2 CCTTGAACGTTTCCAAGTGAG AATCCCTCACCCTAACATAGC 482bp chr11:118175377+118175858
3 TTCTGTTTAGGAATGCAGGTAC ATTTTTCTTTTGTATGGTTAAGACT 317bp chr11:118177937+118178253
4 CAGCTGAGGGAATTGGAAGAT AAATACAGGCAAGTTTCATACG 299bp chr11:118179013+118179311
5 GCTAAATCTCATAGGCTAAGCA AGGGAACTAGAGAACCTTGAAG 466bp chr11:118182691+118183156
6 CCACACCCTCTAGCCAGTAGA TTACCCAGAAGCCTCATATTG 611bp chr11:118183178+118183788
7 GTGGCAAGCGTGTGAGTAGTA TGTGAGATTTGAAACCGTCTG 455bp chr11:118184305+118184759
8 ACAGACAGTGCTGCAAGATTG GAGAAAGAGGAGTATGTCTCCTG 354bp chr11:118185099+118185452
9 CAGTGTCCTTTCTTAGGAGGTAC TAGTCAAAGATAGTGAGAACCTGA 838bp chr11:118186134+118186971

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1-2 CCTTGAACGTTTCCAAGTGAG AATCCCTCACCCTAACATAGC 482bp chr11:118175377+118175858
3 TTCTGTTTAGGAATGCAGGTAC ATTTTTCTTTTGTATGGTTAAGACT 317bp chr11:118177937+118178253
4 CAGCTGAGGGAATTGGAAGAT AAATACAGGCAAGTTTCATACG 299bp chr11:118179013+118179311
5 GCTAAATCTCATAGGCTAAGCA AGGGAACTAGAGAACCTTGAAG 466bp chr11:118182691+118183156
6 CCACACCCTCTAGCCAGTAGA TTACCCAGAAGCCTCATATTG 611bp chr11:118183178+118183788
7 GTGGCAAGCGTGTGAGTAGTA TGTGAGATTTGAAACCGTCTG 455bp chr11:118184305+118184759
8 ACAGACAGTGCTGCAAGATTG GAGAAAGAGGAGTATGTCTCCTG 354bp chr11:118185099+118185452
9 CAGTGTCCTTTCTTAGGAGGTAC TAGTCAAAGATAGTGAGAACCTGA 838bp chr11:118186134+118186971

IV. Ethanol precipitation

V. Sequencing analysis