CD3G
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GTGATGGGTGGAGCCAGTCTA CTGAAGGTTAGGGATACCAATA 245bp chr11:118215044+118215288
2 ACCCTCCCAGGATACCAGGA GAACAGGTTATGTCAGGTCACT 416bp chr11:118219519+118219934
3 CCAGAACTACTAAATAGCACCTG AATTAAGAGAACAGGCGATAATA 518bp chr11:118220295+118220812
4 CCAGATACTTCCTGGTAGTTAGACTA ACATGCTGTGATAACACCAAGTA 536bp chr11:118221038+118221573
5 TCAAGAGTGAGCATTGTCCTGAC CTTCAACTTGTGCTCACTAGATC 503bp chr11:118222091+118222593
6 GTACCCACTCCAAATTCTCAC CTGATGCTTATAGCCGCTTAG 291bp chr11:118223014+118223304

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GTGATGGGTGGAGCCAGTCTA CTGAAGGTTAGGGATACCAATA 245bp chr11:118215044+118215288
2 ACCCTCCCAGGATACCAGGA CTGTTCGCTCCTGTAGGACT 336bp chr11:118219519+118219854
3 CCAGAACTACTAAATAGCACCTG AATTAAGAGAACAGGCGATAATA 518bp chr11:118220295+118220812
4 CCAGATACTTCCTGGTAGTTAGACTA ACATGCTGTGATAACACCAAGTA 536bp chr11:118221038+118221573
5 TCAAGAGTGAGCATTGTCCTGAC CTTCAACTTGTGCTCACTAGATC 503bp chr11:118222091+118222593
6 GTACCCACTCCAAATTCTCAC CTGATGCTTATAGCCGCTTAG 291bp chr11:118223014+118223304

IV. Ethanol precipitation

V. Sequencing analysis