CD40
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ATAGGTGGACCGCGATTGGT AGGCCTCTTCCCCGAAGTCT 263bp chr20:44746855+44747117
2 GCTGAAGAAGTTGCAACGGA AAAGCAAAGGGGGACTCCA 186bp chr20:44750399+44750584
3 GTTAGTGTCTGACTCATGGA AATGGCTGCATTGTCGGGA 250bp chr20:44750813+44751062
4 GGTCTGAGGAAGAAAGAGCA CTTGGGCCCTAAGCTCCT 298bp chr20:44751156+44751453
5 AAGGCAGGAGCTCTTCCCGT GAGGCCACTCTGCAGATGCT 336bp chr20:44751663+44751998
6 GTTGTGTGCTCAGTGAACCT CCTCCCCTTTTTCCCATCCA 294bp chr20:44755145+44755438
7 GTCACAGCAGGTTGAGGGTAG GGCCAGATAAGAAACAGGTGG 219bp chr20:44756708+44756926
8 GTGATCCCCATCATCTTCGGGA GGGGATTGAGCTCTGGATGGA 370bp chr20:44756806+44757175
9a CCGAGGAATCAGCACTGACC AATGGGCATCTGTGTATATGGC 678bp chr20:44757386+44758063

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ATAGGTGGACCGCGATTGGT AGGCCTCTTCCCCGAAGTCT 263bp chr20:44746855+44747117
2 GCTGAAGAAGTTGCAACGGA AAAGCAAAGGGGGACTCCA 186bp chr20:44750399+44750584
3 GTTAGTGTCTGACTCATGGA AATGGCTGCATTGTCGGGA 250bp chr20:44750813+44751062
4 GGTCTGAGGAAGAAAGAGCA CTTGGGCCCTAAGCTCCT 298bp chr20:44751156+44751453
5 AAGGCAGGAGCTCTTCCCGT GAGGCCACTCTGCAGATGCT 336bp chr20:44751663+44751998
6 GTTGTGTGCTCAGTGAACCT CCTCCCCTTTTTCCCATCCA 294bp chr20:44755145+44755438
7 GTCACAGCAGGTTGAGGGTAG GGCCAGATAAGAAACAGGTGG 219bp chr20:44756708+44756926
8 GTGATCCCCATCATCTTCGGGA GGGGATTGAGCTCTGGATGGA 370bp chr20:44756806+44757175
9a CCGAGGAATCAGCACTGACC AATGGGCATCTGTGTATATGGC 678bp chr20:44757386+44758063

IV. Ethanol precipitation

V. Sequencing analysis