CD40LG
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GCTGCAACGATTGTGCGCTC CCATCATTTGGGTAGAACCA 351bp chrX:135730291+135730641
2 TTAGCTGTATTCTCCTTCCG CATTGTCAGTTTCCCGATCT 263bp chrX:135732369+135732631
3 TTAAAACCCCACAGCAGAGC TCCCTGATGCAACAACACTG 214bp chrX:135736473+135736686
4 CAGTTGTAGAACTGGACCAG GGTAACATGACTTCGGCATC 210bp chrX:135738436+135738645
5 TGTGAACCATGCTCTGCTTC GGCTTAACCGCTGTGCTGTA 502bp chrX:135741144+135741645

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GCTGCAACGATTGTGCGCTC CCATCATTTGGGTAGAACCA 351bp chrX:135730291+135730641
2 TTAGCTGTATTCTCCTTCCG CATTGTCAGTTTCCCGATCT 263bp chrX:135732369+135732631
3 TTAAAACCCCACAGCAGAGC TCCCTGATGCAACAACACTG 214bp chrX:135736473+135736686
4 CAGTTGTAGAACTGGACCAG GGTAACATGACTTCGGCATC 210bp chrX:135738436+135738645
5 TGTGAACCATGCTCTGCTTC GGCTTAACCGCTGTGCTGTA 502bp chrX:135741144+135741645

IV. Ethanol precipitation

V. Sequencing analysis