CD79B
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGTTCATTCATAGGACAGGTG CAGGCGGTAAAGAGTGAGAGA 632bp chr17:62009437-62010068
2 TGTCATGGAAGGAGGAAGTAG GACCATAGTCGGACACAGGAC 230bp chr17:62008626-62008855
3 CCTGAGAAGCAAGGACTAAGC ATACCATCCTTCAGCGTGTTC 668bp chr17:62007197-62007864
4-5 GCAGAAGTGCAACAACACCTC CCAACCACACCAGCAGATAGT 773bp chr17:62006723-62007495
6 GAGGAAGATCACACCTACGAG GGCTGGAGACAAATGGCAGCT 375bp chr17:62006440-62006814

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGTTCATTCATAGGACAGGTG CAGGCGGTAAAGAGTGAGAGA 632bp chr17:62009437-62010068
2 TGTCATGGAAGGAGGAAGTAG GACCATAGTCGGACACAGGAC 230bp chr17:62008626-62008855
3 CCTGAGAAGCAAGGACTAAGC ATACCATCCTTCAGCGTGTTC 668bp chr17:62007197-62007864
4-5 GCAGAAGTGCAACAACACCTC CCAACCACACCAGCAGATAGT 773bp chr17:62006723-62007495
6 GAGGAAGATCACACCTACGAG GGCTGGAGACAAATGGCAGCT 375bp chr17:62006440-62006814

IV. Ethanol precipitation

V. Sequencing analysis