NLRP3
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1b CTTCCCGGGTTCAATCAA ACTATGTGCACTTGGCCACAA 739bp chr1:247581691+247582429
2 TGGATACCTGTCCTCTGCAAC GTATGGCCAAGTTACCCAGC 358bp chr1:247586384+247586741
3a TTTCTCCATTCCGGTTACCAC CACAGGTCTCGTGGTGATGAG 763bp chr1:247587042+247587804
3b GTGCCTTTGACGAGCACATAG CTTCAAACGACTCCCTGGAAC 731bp chr1:247587671+247588401
3c GAATCATGGACTGCAGAAGGC AGCTGTGGCAACAGTATTTGG 799bp chr1:247588215+247589013
4 TTTGGTTTCGTGACCCATTTC ATTAGCTGGGTATGGTGGTGG 593bp chr1:247592752+247593344
5 TGTCCTGATGCTTCCTCTGTTC TCACTGAAGCCAGAGTGCG 318bp chr1:247597326+247597643
6 AACTGGAGTAGAGGCAGTGGC TCAGCTCTGCCTGACCAAAG 366bp chr1:247599176+247599541
7 GAGAGGACGAGGCACTGAGTC GATCCTGTAACAAGGCAAACAC 393bp chr1:247607188+247607580
8 CCACCTGAAACAAGACAGGC CAAATGAAGCGAGAGCCATC 360bp chr1:247607859+247608218
9a GTGTGTGGAGTTTAGGGAAATG GGTCACCAAGAGGAACATCC 475bp chr1:247611604+247612078

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1b CTTCCCGGGTTCAATCAA ACTATGTGCACTTGGCCACAA 739bp chr1:247581691+247582429
2 TGGATACCTGTCCTCTGCAAC GTATGGCCAAGTTACCCAGC 358bp chr1:247586384+247586741
3a TTTCTCCATTCCGGTTACCAC CACAGGTCTCGTGGTGATGAG 763bp chr1:247587042+247587804
3b GTGCCTTTGACGAGCACATAG CTTCAAACGACTCCCTGGAAC 731bp chr1:247587671+247588401
3c GAATCATGGACTGCAGAAGGC AGCTGTGGCAACAGTATTTGG 799bp chr1:247588215+247589013
4 TTTGGTTTCGTGACCCATTTC ATTAGCTGGGTATGGTGGTGG 593bp chr1:247592752+247593344
5 TGTTCTGATGCTTTCTGCCTC TCACTGAAGCCAGAGTGCG 276bp chr1:247597368+247597643
6 AACTGGAGTAGAGGCAGTGGC TCAGCTCTGCCTGACCAAAG 366bp chr1:247599176+247599541
7 GAGAGGACGAGGCACTGAGTC GATCCTGTAACAAGGCAAACAC 393bp chr1:247607188+247607580
8 CCACCTGAAACAAGACAGGC CAAATGAAGCGAGAGCCATC 360bp chr1:247607859+247608218
9a GTGTGTGGAGTTTAGGGAAATG GGTCACCAAGAGGAACATCC 475bp chr1:247611604+247612078

IV. Ethanol precipitation

V. Sequencing analysis