CSF3R
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 GCTCAGAGAAGTCAAGCAACC TGGCTCAGACTTGAATCTTGTG 320bp chr1:36944905-36945224
4 TAGCCTCTCGAAGTCTCCGTC CTTCCTGGGTTCGAGCAGTTC 747bp chr1:36940693-36941439
7 CAACGTGGACAGATGGTGAAC GACTCTGAGCCTTGGATCGC 382bp chr1:36938011-36938392
10 GAGGCTCCGTTTGTATGATCC CCGGCCAATAGGACTAGATTTAAC 405bp chr1:36936942-36937346
11 CAAGAACTACTCCATGCCAGG TGGAGAATCCATGTCTCTTGG 553bp chr1:36935145-36935697
12 CCCTCACTAGAGGTCTGAGGC ATGGTAGGAAGGCAATGTTCC 293bp chr1:36934652-36934944
5-6 GGTGACAACAGAGGAACCAAG TTCTTGTCTGTCGCTAGTTCTGTG 656bp chr1:36938945-36939600
8-9 AACCTGGAGAGCCTGAGGTG CAGACCTGTTGGAGTCCTAAGC 530bp chr1:36937574-36938103
13-14 ATGAGGCTTGGGCATGAGTC TAGAATGCATGTTGGGAAGGC 675bp chr1:36933281-36933955
15-16 CAGAGATGGCTGTTTGGAGG GAGTACTTGGCTTCAGAAGGTGTC 654bp chr1:36932747-36933400
17a ATGCTGAGTCTGAACGGTGC CCCAAAGACACAGTCGTCCTC 598bp chr1:36932027-36932624
17b GGCACTATCTCCGCTGTGAC GGGAGGGAGGAAGAAAGGAC 613bp chr1:36931563-36932175

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 GCTCAGAGAAGTCAAGCAACC TGGCTCAGACTTGAATCTTGTG 320bp chr1:36944905-36945224
4 TAGCCTCTCGAAGTCTCCGTC CTTCCTGGGTTCGAGCAGTTC 747bp chr1:36940693-36941439
7 CAACGTGGACAGATGGTGAAC GACTCTGAGCCTTGGATCGC 382bp chr1:36938011-36938392
10 GAGGCTCCGTTTGTATGATCC CCGGCCAATAGGACTAGATTTAAC 405bp chr1:36936942-36937346
11 CAAGAACTACTCCATGCCAGG TGGAGAATCCATGTCTCTTGG 553bp chr1:36935145-36935697
12 CCCTCACTAGAGGTCTGAGGC ATGGTAGGAAGGCAATGTTCC 293bp chr1:36934652-36934944
5-6 GGTGACAACAGAGGAACCAAG TTCTTGTCTGTCGCTAGTTCTGTG 656bp chr1:36938945-36939600
8-9 AACCTGGAGAGCCTGAGGTG CAGACCTGTTGGAGTCCTAAGC 530bp chr1:36937574-36938103
13-14 ATGAGGCTTGGGCATGAGTC TAGAATGCATGTTGGGAAGGC 675bp chr1:36933281-36933955
15-16 CAGAGATGGCTGTTTGGAGG GAGTACTTGGCTTCAGAAGGTGTC 654bp chr1:36932747-36933400
17a ATGCTGAGTCTGAACGGTGC CCCAAAGACACAGTCGTCCTC 598bp chr1:36932027-36932624
17b GGCACTATCTCCGCTGTGAC GGGAGGGAGGAAGAAAGGAC 613bp chr1:36931563-36932175

IV. Ethanol precipitation

V. Sequencing analysis