CXCR4
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1a CCTGAAGAAAGCAAGCCTGAATTG TGGAACACAACCACCCACAAG 697bp chr2:136872899-136873595
1b GTCCACGCCACCAACAGTCAG CACAGGTCCTGCCTAGACACACATC 854bp chr2:136872230-136873083

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1a CCTGAAGAAAGCAAGCCTGAATTG TGGAACACAACCACCCACAAG 697bp chr2:136872899-136873595
1b GTCCACGCCACCAACAGTCAG CACAGGTCCTGCCTAGACACACATC 854bp chr2:136872230-136873083

IV. Ethanol precipitation

V. Sequencing analysis