CYBA
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ACCCTACAGATGCGGGTCTC CCCTGTAAGTAGCCCGAGGTC 279bp chr16:88717280-88717558
2 CCACAGGCTCTGCTCTTCAC CCACCACTCACCAGGACTTAG 486bp chr16:88714226-88714711
3 CCTCTTACCTCAGCCTCTGG CCACGGCGGTCATGTACTTC 638bp chr16:88713221-88713858
4 TGGAGCTTGGTTTCTCACTTG GTAACCAGCAGACACACAGCA 592bp chr16:88712849-88713440
5 CAGTCTGCCTTGTCCTGAGAC AGGCTCACACTTGCTCCCAG 320bp chr16:88712394-88712713
6 AGCCTGTCCCAGGGCCCCT GCTCACGCGCTCCCGGCTT 392bp chr16:88709687-88710078

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CGGCAGTGCGCGCCTA CCCTGTAAGTAGCCCGAGGTC 186bp chr16:88717280-88717465
2 CCACAGGCTCTGCTCTTCAC CCACCACTCACCAGGACTTAG 486bp chr16:88714226-88714711
3 CCTCTTACCTCAGCCTCTGG CCACGGCGGTCATGTACTTC 638bp chr16:88713221-88713858
4 TGGAGCTTGGTTTCTCACTTG GTAACCAGCAGACACACAGCA 592bp chr16:88712849-88713440
5 CAGTCTGCCTTGTCCTGAGAC AGGCTCACACTTGCTCCCAG 320bp chr16:88712394-88712713
6 AGCCTGTCCCAGGGCCCCT GCTCACGCGCTCCCGGCTT 392bp chr16:88709687-88710078

IV. Ethanol precipitation

V. Sequencing analysis