FOXP3
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 TGGCTCAGGTGGTCGAGTATC CCCAGTGCCACAGTAAAGGTC 383bp chrX:49114673-49115055
3-4 CATCTACCATGTGGGCTTGC CTACACTGCTCACAGCCAAGG 511bp chrX:49113810-49114320
5-6 AGCTTCTCTGCCAGAAGCCTG AGCTCTTGCATCTTACTACTTATTGG 534bp chrX:49113098-49113631
7-8 AGAGCATTGAGCCAGACCAG TGAGGTTAGGTTCCCTGCAC 521bp chrX:49111804-49112324
9 GCAGAAGACTTGCTTGAATCTG ATGGCCGAATATAGTAGCTGGAG 619bp chrX:49110088-49110706
10 CAGGCCTGTAGACTCACCTTG GGAAGGAGTTAGAGTAAGAGCTGG 543bp chrX:49109212-49109754
11 GTCCTGTCCCTGATTACCTGC AATGTGCCTATGAGCCCAGAC 319bp chrX:49108032-49108350
12a CTGGGCTCATAGGCACATTG TTGTGAAGGCTCTGTTTGGC 545bp chrX:49107506-49108050

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 TGGCTCAGGTGGTCGAGTATC CCCAGTGCCACAGTAAAGGTC 383bp chrX:49114673-49115055
3-4 CATCTACCATGTGGGCTTGC CTACACTGCTCACAGCCAAGG 511bp chrX:49113810-49114320
5-6 AGCTTCTCTGCCAGAAGCCTG AGCTCTTGCATCTTACTACTTATTGG 534bp chrX:49113098-49113631
7-8 CCAGACCAGGCCTGTCGT TGCCCCCCAGCAGTCTGA 473bp chrX:49111841-49112313
9 GCAGAAGACTTGCTTGAATCTG ATGGCCGAATATAGTAGCTGGAG 619bp chrX:49110088-49110706
10 CAGGCCTGTAGACTCACCTTG GGAAGGAGTTAGAGTAAGAGCTGG 543bp chrX:49109212-49109754
11 GTCCTGTCCCTGATTACCTGC AATGTGCCTATGAGCCCAGAC 319bp chrX:49108032-49108350
12a CTGGGCTCATAGGCACATTG TTGTGAAGGCTCTGTTTGGC 545bp chrX:49107506-49108050

IV. Ethanol precipitation

V. Sequencing analysis