GFI1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CAAGTGCCACCTGGTAAGCG ACCTAGGAGACAAACCCGGAG 533bp chr1:92948772-92949304
3 GGTTCCTCCGGGTTTGTCTC TACGAATCAGCTCCCTTCTCC 514bp chr1:92948284-92948797
4 GCACGCAGTGTTCTACAAGC AAGGGTCTGGTACCGCTGTG 795bp chr1:92945978-92946772
5 CTCCTACAAGTGCATCAAGTGC AACTTCCAGGCAGAGAGTGGC 372bp chr1:92945814-92946185
6 TGAGGAGAGCAGCAGTCTAGTTG AAGGCCTCATCCACCACTCAC 348bp chr1:92944047-92944394
7a GAAGGCCTTAGACTGTGGTGC AAACCTGAAGGGCATTCCTG 661bp chr1:92941220-92941880

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CAAGTGCCACCTGGTAAGCG ACCTAGGAGACAAACCCGGAG 533bp chr1:92948772-92949304
3 GGTTCCTCCGGGTTTGTCTC TACGAATCAGCTCCCTTCTCC 514bp chr1:92948284-92948797
4 GCACGCAGTGTTCTACAAGC AAGGGTCTGGTACCGCTGTG 795bp chr1:92945978-92946772
5 CTCCTACAAGTGCATCAAGTGC AACTTCCAGGCAGAGAGTGGC 372bp chr1:92945814-92946185
6 CTCTCTCTCTCTCTGCAGGA AAGGCCTCATCCACCACTCAC 282bp chr1:92944047-92944328
7a GAAGGCCTTAGACTGTGGTGC AAACCTGAAGGGCATTCCTG 661bp chr1:92941220-92941880

IV. Ethanol precipitation

V. Sequencing analysis