HAX1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AAGAAGAACAGATTTAGGAGGAA GCTTAGTTTGCTGTTTCTCCT 553bp chr1:154244893+154245445
2 ATTGTATTAATGTTTTGATGTGG AATCATCAGTCTTCTCACACTTC 526bp chr1:154245660+154246185
3 CCTAGTACGAGATTTCAATAGCA CACCTAGCCAGTCTTTGCACT 504bp chr1:154246007+154246510
4-5 GCTCGGGAGTAGTTTGAGGTC TGTTTCAGGGCATTCGTTC 451bp chr1:154247344+154247794
6-7 GGATCTGTGTAAAGAAACTGCTG TCGGAAGTGTTGGATGGTTC 615bp chr1:154247796+154248410

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AAGAAGAACAGATTTAGGAGGAA GCTTAGTTTGCTGTTTCTCCT 553bp chr1:154244893+154245445
2 ATTGTATTAATGTTTTGATGTGG AATCATCAGTCTTCTCACACTTC 526bp chr1:154245660+154246185
3 CCTAGTACGAGATTTCAATAGCA CACCTAGCCAGTCTTTGCACT 504bp chr1:154246007+154246510
4-5 GCTCGGGAGTAGTTTGAGGTC TGTTTCAGGGCATTCGTTC 451bp chr1:154247344+154247794
6-7 GGATCTGTGTAAAGAAACTGCTG TCGGAAGTGTTGGATGGTTC 615bp chr1:154247796+154248410

IV. Ethanol precipitation

V. Sequencing analysis