IFNGR1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCTTTGCACTCAAATTCCTCC AACCGACGAGTTCAAACCAC 534bp chr6:137540187-137540720
2 TACATATCTGGGCAATGTGGC GGAAGGCTGATGAAAGAACAC 300bp chr6:137528011-137528310
3 GCAAAGCACAGACAGAAATGG ACTGACTCTAAATTCCTCCAAGTG 385bp chr6:137527138-137527522
4 GGTCCTGCTTTAGAACAACCAG TCAAATCTATGATCTGTGAGTCTTGC 424bp chr6:137525343-137525766
5 TGTTTGACCAGGACTAATATGGTG TTTGCTTTCCTCACATGATCC 347bp chr6:137524563-137524909
6 TCAGATTTCCTAACTTCTGCTTCC GACTGATTGATGGCAGGTGAC 406bp chr6:137521895-137522300
7a AAATGTGCCATTTGGTGGTC CGGTTATGAGCTCTTGTCCTTC 585bp chr6:137519296-137519880
7b GGCAGCATCGCTTTAAACTC TGCCACACATCCTCTTTACGCT 461bp chr6:137519010-137519470

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCTTTGCACTCAAATTCCTCC AACCGACGAGTTCAAACCAC 534bp chr6:137540187-137540720
2 TACATATCTGGGCAATGTGGC GGAAGGCTGATGAAAGAACAC 300bp chr6:137528011-137528310
3 GCAAAGCACAGACAGAAATGG ACTGACTCTAAATTCCTCCAAGTG 385bp chr6:137527138-137527522
4 GGTCCTGCTTTAGAACAACCAG TCAAATCTATGATCTGTGAGTCTTGC 424bp chr6:137525343-137525766
5 CAGATTAAAAGAAGCTGTGCA TTTGCTTTCCTCACATGATCC 308bp chr6:137524563-137524870
6 AAACTCTGGCCCTATTCACT CATCTTGTTGAATATCATTC 348bp chr6:137521915-137522262
7a AAATGTGCCATTTGGTGGTC CGGTTATGAGCTCTTGTCCTTC 585bp chr6:137519296-137519880
7b GGCAGCATCGCTTTAAACTC TGCCACACATCCTCTTTACGCT 461bp chr6:137519010-137519470

IV. Ethanol precipitation

V. Sequencing analysis