IGLL1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGTGCAATGGGACAGCTGCT CTCCTCACCCCTCTGGCT 401bp chr22:23922080-23922480
2 TGGGTCACAGCCTACACACT CAGAAGAGGGTGGGACAGC chr22:23917298-?
3 AGACAGAGAGAGGCGCTCCA GGATGCAGAGAGAGACCCTT 465bp chr22:23915380-23915844

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGTGCAATGGGACAGCTGCT CTCCTCACCCCTCTGGCT 401bp chr22:23922080-23922480
2 TGGGTCACAGCCTACACACT CAGAAGAGGGTGGGACAGC chr22:23917298-?
3 AGACAGAGAGAGGCGCTCCA GGATGCAGAGAGAGACCCTT 465bp chr22:23915380-23915844

IV. Ethanol precipitation

V. Sequencing analysis