IKBKG
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CAGCAAGAATCTTATCAGACCAATG CTCCTCCCTGGCCAGCTTA 467bp chrX:153770348+153770814
2 TTCTGCTGGGTAAGGATGTG TCCTGAAACCAAGAGGGAGG 378bp chrX:153780146+153780523
3 GAAGCTGCGTGGTATTATGGC GCTCACCGTAACCTGTGTCC 551bp chrX:153784228+153784778
4 TAAGCCGTTGACAACCTCAAG CGGTCTATCCTCATCAAGGAGC 519bp chrX:153786448+153786966
5 TGTCCTCGTGTCTGTTGACG GCCACAGGCAGAATCTGAAG 444bp chrX:153788462+153788905
6 ACCCCATTCCAGGCCTGTGAT ATTCTCAAGGTTCAGGGCCAC 287bp chrX:153789785+153790071
7 AGGGCCACTCTTTCATCCTTC AGACGCCAAAGAGACTCTCCAG 294bp chrX:153790936+153791229
8 GTGAGCTTGGCATTACTCGC CCAGAACACCAGGAGCCATC 439bp chrX:153791614+153792052
9 ACACTAGTCCAGCCAAGGAGC CAACAGGAAGGTCGAACTGTG 218bp chrX:153792108+153792325
10a AGTCCTGCCAAGTCTCCGTC AGGAAGAGGGAGGGATTAGCC 495bp chrX:153792471+153792965

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CAGCAAGAATCTTATCAGACCAATG CTCCTCCCTGGCCAGCTTA 467bp chrX:153770348+153770814
2 TTCTGCTGGGTAAGGATGTG TCCTGAAACCAAGAGGGAGG 378bp chrX:153780146+153780523
3 GAAGCTGCGTGGTATTATGGC GCTCACCGTAACCTGTGTCC 551bp chrX:153784228+153784778
4 TAAGCCGTTGACAACCTCAAG CGGTCTATCCTCATCAAGGAGC 519bp chrX:153786448+153786966
5 TGTCCTCGTGTCTGTTGACG GCCACAGGCAGAATCTGAAG 444bp chrX:153788462+153788905
6 ACCCCATTCCAGGCCTGTGAT ATTCTCAAGGTTCAGGGCCAC 287bp chrX:153789785+153790071
7 AGGGCCACTCTTTCATCCTTC AGACGCCAAAGAGACTCTCCAG 294bp chrX:153790936+153791229
8 GTGAGCTTGGCATTACTCGC CCAGAACACCAGGAGCCATC 439bp chrX:153791614+153792052
9 ACACTAGTCCAGCCAAGGAGC CAACAGGAAGGTCGAACTGTG 218bp chrX:153792108+153792325
10a AGTCCTGCCAAGTCTCCGTC AGGAAGAGGGAGGGATTAGCC 495bp chrX:153792471+153792965

IV. Ethanol precipitation

V. Sequencing analysis