IL12B
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CAAAGGTAACAAGAATTATTGACATTC GCCCATTTCAGTTTGATTTCC 279bp chr5:158753612-158753890
3 CAAGGTTGTGTTTAGGCAATGG AAGGGTCCAGGAAAGCAAAG 553bp chr5:158749888-158750440
4 ACGCATGGAAGAACTTCACC TGATAGGTCACTGAGAGGTTGC 313bp chr5:158749318-158749630
5 TTGAGGCCTTACCTGCCTTAC TGATGCTTGTCAACCACCTACC 404bp chr5:158747213-158747616
6 AACTATCCAGTGGACTCACAGC TGTTAAGTGATACATGGATGTCATTG 381bp chr5:158745633-158746013
7 GTATGCATTGCAGAAACAGGC CCTTTCCTCTCCAACACAGC 304bp chr5:158743602-158743905

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CAAAGGTAACAAGAATTATTGACATTC GCCCATTTCAGTTTGATTTCC 279bp chr5:158753612-158753890
3 CAAGGTTGTGTTTAGGCAATGG AAGGGTCCAGGAAAGCAAAG 553bp chr5:158749888-158750440
4 ACGCATGGAAGAACTTCACC TGATAGGTCACTGAGAGGTTGC 313bp chr5:158749318-158749630
5 TTGAGGCCTTACCTGCCTTAC TGATGCTTGTCAACCACCTACC 404bp chr5:158747213-158747616
6 AACTATCCAGTGGACTCACAGC TGTTAAGTGATACATGGATGTCATTG 381bp chr5:158745633-158746013
7 GTATGCATTGCAGAAACAGGC CCTTTCCTCTCCAACACAGC 304bp chr5:158743602-158743905

IV. Ethanol precipitation

V. Sequencing analysis