BTK
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 GGGAACCAAGAGGGATGAGG CCAAGAAAGATCTAAGGCCAAGTC 297bp chrX:100630066-100630362
3 GTCCTGGTGCCACCTCACTT GCATCACCAGTCTATTTACAG 296bp chrX:100629436-100629731
4 GAGAAACTTCTGAAGCCACAGC ACTGGGTCCTCGTAGCCTTC 268bp chrX:100626523-100626790
5 TAACCTGAACACCATTGCTGAC CCGTCTATCTCCTCTTCCTTC 298bp chrX:100624919-100625216
6 CAGGCAGGCCAGTCATGTGA AGTGATGGAAACAGTCAAAGG 350bp chrX:100617497-100617846
7 CCAGCTGGTAATCTGCTCTCC CCAATTAACACTGCCAAGTCC 342bp chrX:100617052-100617393
8 TGGGTGTCTTGAGTAAGCCAG GCTGATCACGGGAATTATGC 366bp chrX:100615470-100615835
9 GATTGTCTTTGAGGGAGGTGC TCCTGGAAGATTGTGGACTGAC 236bp chrX:100615040-100615275
10 TGAATCACTGACATGGACAAGC AGACGATGGCAGCTTTGACAC 262bp chrX:100614166-100614427
11-12 GTAGCTCAAGCGTGGAACTTG CCTTGTCCTGCATTGCTTATC 578bp chrX:100613235-100613812
13 TACACACCGGAAGTGTGAGGC CTTGGATCTGTCTTGAGCGTC 304bp chrX:100612361-100612664
14 GAATCCCGTTTCTGAGATTGAC TGTAAGTCATCTTTAAGCCTCAGTG 422bp chrX:100611682-100612103
15 CCCTTTATTGCTATTATGCAGAGC AAATTGAAATGATGGCACCAG 388bp chrX:100610929-100611316
16 CCACGTGGTGGAAATGAATC GAGAAAGGAAGGAAAGGGATATG 458bp chrX:100609407-100609864
17 CGTAAGCAAATATCAAGCCTCC TTCCCATTGCATTTCTTATCC 234bp chrX:100608800-100609033
18 CCAACTGATTCTAATCTTTGAGGTTG TCAGTCTTTGGTGGCTGAATG 339bp chrX:100608099-100608437
19 GCTGCTTACTCATTGCATTTCC TGCCAAACTAATGCTACACAGC 670bp chrX:100604350-100605019

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 GGGAACCAAGAGGGATGAGG CCAAGAAAGATCTAAGGCCAAGTC 297bp chrX:100630066-100630362
3 GTCCTGGTGCCACCTCACTT GCATCACCAGTCTATTTACAG 296bp chrX:100629436-100629731
4 GAGAAACTTCTGAAGCCACAGC ACTGGGTCCTCGTAGCCTTC 268bp chrX:100626523-100626790
5 TAACCTGAACACCATTGCTGAC CCGTCTATCTCCTCTTCCTTC 298bp chrX:100624919-100625216
6 CAGGCAGGCCAGTCATGTGA AGTGATGGAAACAGTCAAAGG 350bp chrX:100617497-100617846
7 CCAGCTGGTAATCTGCTCTCC CCAATTAACACTGCCAAGTCC 342bp chrX:100617052-100617393
8 TGGGTGTCTTGAGTAAGCCAG GCTGATCACGGGAATTATGC 366bp chrX:100615470-100615835
9 GATTGTCTTTGAGGGAGGTGC TCCTGGAAGATTGTGGACTGAC 236bp chrX:100615040-100615275
10 TGAATCACTGACATGGACAAGC AGACGATGGCAGCTTTGACAC 262bp chrX:100614166-100614427
11-12 GTAGCTCAAGCGTGGAACTTG CCTTGTCCTGCATTGCTTATC 578bp chrX:100613235-100613812
13 TACACACCGGAAGTGTGAGGC CTTGGATCTGTCTTGAGCGTC 304bp chrX:100612361-100612664
14 GAATCCCGTTTCTGAGATTGAC TGTAAGTCATCTTTAAGCCTCAGTG 422bp chrX:100611682-100612103
15 CCCTTTATTGCTATTATGCAGAGC AAATTGAAATGATGGCACCAG 388bp chrX:100610929-100611316
16 CCACGTGGTGGAAATGAATC GAGAAAGGAAGGAAAGGGATATG 458bp chrX:100609407-100609864
17 CGTAAGCAAATATCAAGCCTCC TTCCCATTGCATTTCTTATCC 234bp chrX:100608800-100609033
18 CCAACTGATTCTAATCTTTGAGGTTG TCAGTCTTTGGTGGCTGAATG 339bp chrX:100608099-100608437
19 GCTGCTTACTCATTGCATTTCC TGCCAAACTAATGCTACACAGC 670bp chrX:100604350-100605019

IV. Ethanol precipitation

V. Sequencing analysis