IL12RB1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TTTGACTTGCCTTAGGGATGG ACAGCTCTCCACACATACACG 305bp chr19:18197500-18197804
2 GCTGATGATCACTTGGGTGTAG GGGCAACTTGAACCTGGTAAG 330bp chr19:18194067-18194396
3 CACAAAGATTTGTCTTAGGCACG ATCACGCATCCGAGAGTAGG 279bp chr19:18192870-18193148
4 GGATGTGATGCACAAAGTCG TCCTCTCTAGCTCCAGCCTTG 323bp chr19:18191566-18191888
5 GGGCCCATTAACTCACATACG GAGACTGATTTGGGGGTGAAG 270bp chr19:18188256-18188525
6 CTGGGAGGTTGAGGCTTCAA AGCATTGGACCTGGCATACAG 284bp chr19:18187024-18187307
7 GGCTCTGTACACAGTTGGAGC CTCCGGCTGTTGCCTTATTTC 284bp chr19:18186499-18186782
8 GGCAGCAAGGGATTTTCAC CTAGGCTTTTGCCTCCTGCTC 259bp chr19:18184264-18184522
9 TCTGTCTTCATAGGCTGTGCC CTCCTCTCACCCTGGTCTAGC 415bp chr19:18182834-18183248
10 ATAGACGAGAGGGTGGGAGAG AACCCACCAGGACCTAAAAGG 332bp chr19:18180290-18180621
11 GTTCCTGGCAGATGGCAC TGTCTGCCTTAACAGCATTGTC 619bp chr19:18178805-18179423
12 TGCTGGGATTACAGGTGCGA CCCAGAGAAGGACGGCAAG 341bp chr19:18177250-18177590
13 TGGCCTCTGAGGAGTAAAGAG CTAAGATCATTGTGGGAAGCG 261bp chr19:18174615-18174875
14 ATCAGCAGCAAGTTCATGGAG CTCTCAGCGGCTACTCTCCG 421bp chr19:18172766-18173186
15 ACTGGTGGGCATGTCTGATTG TAGTGCCAGGAAATGGACCTC 618bp chr19:18171756-18172373
16 AATTTAACCAGCAGTGACCTGG GATATCCCAGGAGCGATGG 371bp chr19:18170617-18170987
17 AGGCCTGAGGGAGAAACGAA GGGTCCAAATGTGACTCCTGT 262bp chr19:18170346-18170607

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TTTGACTTGCCTTAGGGATGG ACAGCTCTCCACACATACACG 305bp chr19:18197500-18197804
2 GCTGATGATCACTTGGGTGTAG GGGCAACTTGAACCTGGTAAG 330bp chr19:18194067-18194396
3 CACAAAGATTTGTCTTAGGCACG ATCACGCATCCGAGAGTAGG 279bp chr19:18192870-18193148
4 GGATGTGATGCACAAAGTCG TCCTCTCTAGCTCCAGCCTTG 323bp chr19:18191566-18191888
5 GGGCCCATTAACTCACATACG GAGACTGATTTGGGGGTGAAG 270bp chr19:18188256-18188525
6 CTGGGAGGTTGAGGCTTCAA AGCATTGGACCTGGCATACAG 284bp chr19:18187024-18187307
7 GGCTCTGTACACAGTTGGAGC CTCCGGCTGTTGCCTTATTTC 284bp chr19:18186499-18186782
8 GTGGAGAATCCATCATTTAC TCCTGCTCCCCATCCCA 218bp chr19:18184277-18184494
9 TCTGTCTTCATAGGCTGTGCC TCAGAGTAGGTGCTCAGCT 388bp chr19:18182861-18183248
10 ATAGACGAGAGGGTGGGAGAG AACCCACCAGGACCTAAAAGG 332bp chr19:18180290-18180621
11 GCACAGCCAGTGCAAAGGT TGTCACCCAGACTGGAGTACA 539bp chr19:18178871-18179409
12 TGCGAGCCACTGTGCCA AGGTGCCCAGGGTCACACA chr19:?-18177285
13 TGGCCTCTGAGGAGTAAAGAG CTAAGATCATTGTGGGAAGCG 261bp chr19:18174615-18174875
14 ATCAGCAGCAAGTTCATGGAG CTCTCAGCGGCTACTCTCCG 421bp chr19:18172766-18173186
15 AACCTCAGCTCCTCTCACTG TAGTGCCAGGAAATGGACCTC 290bp chr19:18171756-18172045
16 AATTTAACCAGCAGTGACCTGG GATATCCCAGGAGCGATGG 371bp chr19:18170617-18170987
17 AGGCCTGAGGGAGAAACGAA GGGTCCAAATGTGACTCCTGT 262bp chr19:18170346-18170607

IV. Ethanol precipitation

V. Sequencing analysis