LIG4
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2a AACTTCAAATTAGGGTTGGAGC TTCCAGATCTGTAGTGACATTATGC 893bp chr13:108862951-108863843
2b TGTTGAAGCCAAGATGTTTACAG GGCCATCATCTCACCATCAAG 608bp chr13:108862615-108863222
2c TCTCTCGAAATGGATATAACTACACTG GAAACACAGATGGCTTCTCACC 630bp chr13:108862116-108862745
2d TCAGTGGACTAATGGATGAATTG GGCTGGCTATCTGTTCCACTC 658bp chr13:108861596-108862253
2e TGGTAAGCTCGCATCTAAACAC GTGGCGTCGAAACATACTGAG 628bp chr13:108861169-108861796
2f GTGCCCATCAACCAAAGAAC TACCACCTGCTGCAATGAGTC 558bp chr13:108860822-108861379

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2a AACTTCAAATTAGGGTTGGAGC TTCCAGATCTGTAGTGACATTATGC 893bp chr13:108862951-108863843
2b TGTTGAAGCCAAGATGTTTACAG GGCCATCATCTCACCATCAAG 608bp chr13:108862615-108863222
2c TCTCTCGAAATGGATATAACTACACTG GAAACACAGATGGCTTCTCACC 630bp chr13:108862116-108862745
2d TCAGTGGACTAATGGATGAATTG GGCTGGCTATCTGTTCCACTC 658bp chr13:108861596-108862253
2e TGGTAAGCTCGCATCTAAACAC GTGGCGTCGAAACATACTGAG 628bp chr13:108861169-108861796
2f GTGCCCATCAACCAAAGAAC TACCACCTGCTGCAATGAGTC 558bp chr13:108860822-108861379

IV. Ethanol precipitation

V. Sequencing analysis