LRRC8A
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3a CTCCCAGCTAGATTTGAGGTG CAGCAGGATAGACACAAAGTG 618bp chr9:131669309+131669926
3b GTGTGCTATGAGAACCGACTG GATGAGGCCGTAGAAGATGAC 672bp chr9:131669777+131670448
3c TCTGCTACACCGTCTACTACG ATCTCCTTGATGTCGGTGAAC 688bp chr9:131670279+131670966
3d CTCAAGGAGCTGTGGCTCTAC AGCTTCTATCCTGGATGGTTC 903bp chr9:131670854+131671756
4a AACAGATGGAAGGATGAGCAC CCCACAGATACTGTCCTCTCG 714bp chr9:131678108+131678821
4b GAGGACCTGTTCAACACACTG ACTGCCACAAATACTGAAGAG 719bp chr9:131678579+131679297

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3a CTCCCAGCTAGATTTGAGGTG CAGCAGGATAGACACAAAGTG 618bp chr9:131669309+131669926
3b GTGTGCTATGAGAACCGACTG GATGAGGCCGTAGAAGATGAC 672bp chr9:131669777+131670448
3c TCTGCTACACCGTCTACTACG ATCTCCTTGATGTCGGTGAAC 688bp chr9:131670279+131670966
3d CTCAAGGAGCTGTGGCTCTAC AGCTTCTATCCTGGATGGTTC 903bp chr9:131670854+131671756
4a AACAGATGGAAGGATGAGCAC CCCACAGATACTGTCCTCTCG 714bp chr9:131678108+131678821
4b GAGGACCTGTTCAACACACTG ACTGCCACAAATACTGAAGAG 719bp chr9:131678579+131679297

IV. Ethanol precipitation

V. Sequencing analysis