MEFV
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CTGTAAGAAGAGAACACAGCCG GCTGCTCTGAGCTCCTGGTC 547bp chr16:3306229-3306775
2a CTCTCTCCTCTGCCCTGAATC AAGGGCCTGCACTCCTTC 484bp chr16:3304382-3304865
2b GCAGAGAGAAGGCCTCGG AATTTCGTTTATAGAGATGGCGG 612bp chr16:3303975-3304586
3 AGAACTCGCACATCTCAGGC CCAAGAATGCTGGTTAATGCAC 569bp chr16:3299320-3299888
4 GTATGGCTGAATCCAGAGGC TCTACCATCTTCTGGTGAGTATGAG 561bp chr16:3298665-3299225
5 CCACCTCTTATCCACCTCCAG TCACCAAGACCAAGTCCTATCC 470bp chr16:3296880-3297349
6 GTGCTCCACTTCCACTGACAC GGCTTAGCCTCTTCCTGTCC 593bp chr16:3296103-3296695
7 CCAGTACATGACAGTCTGTGGC GATGGAGGAGAGGTTGAAGGC 436bp chr16:3294345-3294780
8 ACCTGCCTTCAACCTCTCCTC CCTCAAGTCAACAGCACAAGG 187bp chr16:3294183-3294369
9 TTCCTTGTTGTCAGGACAGTG GCATCCAGAATCACATTAACTGC 301bp chr16:3293676-3293976
10a AGAACCCTGTAGGGATGTTGC AGCTGTGTTCTTCCCTCCATC 572bp chr16:3293183-3293754
10b ACCCGCCTGCTAATAAAGGAG CACAAATACTTCTATCCCAATCCAG 656bp chr16:3292713-3293368

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CTGTAAGAAGAGAACACAGCCG GCTGCTCTGAGCTCCTGGTC 547bp chr16:3306229-3306775
2a CTCTCTCCTCTGCCCTGAATC AAGGGCCTGCACTCCTTC 484bp chr16:3304382-3304865
2b GCAGAGAGAAGGCCTCGG AATTTCGTTTATAGAGATGGCGG 612bp chr16:3303975-3304586
3 AGAACTCGCACATCTCAGGC CCAAGAATGCTGGTTAATGCAC 569bp chr16:3299320-3299888
4 GTATGGCTGAATCCAGAGGC TCTACCATCTTCTGGTGAGTATGAG 561bp chr16:3298665-3299225
5 CCACCTCTTATCCACCTCCAG TCACCAAGACCAAGTCCTATCC 470bp chr16:3296880-3297349
6 GTGCTCCACTTCCACTGACAC GGCTTAGCCTCTTCCTGTCC 593bp chr16:3296103-3296695
7 CCAGTACATGACAGTCTGTGGC GATGGAGGAGAGGTTGAAGGC 436bp chr16:3294345-3294780
8 ACCTGCCTTCAACCTCTCCTC CCTCAAGTCAACAGCACAAGG 187bp chr16:3294183-3294369
9 TTCCTTGTTGTCAGGACAGTG GCATCCAGAATCACATTAACTGC 301bp chr16:3293676-3293976
10a AGAACCCTGTAGGGATGTTGC AGCTGTGTTCTTCCCTCCATC 572bp chr16:3293183-3293754
10b ACCCGCCTGCTAATAAAGGAG CACAAATACTTCTATCCCAATCCAG 656bp chr16:3292713-3293368

IV. Ethanol precipitation

V. Sequencing analysis