Forward sequence | Reverse sequence | |||
2 | TAGCAAGTGCTGGACGGTAAC | TTGCTATCATACCGAGCGAG | 413bp | chr12:110012486+110012898 |
3 | GCCAAGGTTGATTAGCAGTGTC | GCCTTGATGATCTGTCCACAAC | 512bp | chr12:110013662+110014173 |
4 | CCATGTTCCAATTCCAGTGAC | GAGAGCATGTGCATTCTCCAG | 346bp | chr12:110017502+110017847 |
5 | CTGGACCAGATGCTTGGAGTC | GGAGGTGGTGAGGTCTCAGTG | 345bp | chr12:110019096+110019440 |
6 | TCCGTAGCTGGAGAGGTTCAG | TCCTGAACCTACAACTCTCTTGG | 519bp | chr12:110023674+110024192 |
7 | CTGGCAAGAGCTTCCTGGTG | GGAGAAGGAGAGAGCAGGTC | 434bp | chr12:110024360+110024793 |
8 | GACTGCTCCCAGTCCTGTCC | CCCTATAACCCATCAGCCAGAG | 259bp | chr12:110028504+110028762 |
9 | GGCTGTGTGAACACCTCCTC | AGCACAGCCAGATTGCAGAG | 298bp | chr12:110028945+110029242 |
10 | TGTCTCCAGCCAACAACTGTC | TTCAAGGGAATTCTCCAGGTG | 334bp | chr12:110032727+110033060 |
11a | CTTTTGCCTTGAATATGATGAGC | AGAATAATCCAGAAAGGGGCATC | 281bp | chr12:110034155+110034435 |
Exon No. | Forward sequence | Reverse sequence | Expected PCR product size (bp) |
Genomic regions amplified by ePCR with the primer |
2 | TAGCAAGTGCTGGACGGTAAC | TTGCTATCATACCGAGCGAG | 413bp | chr12:110012486+110012898 |
3 | GCCAAGGTTGATTAGCAGTGTC | GCCTTGATGATCTGTCCACAAC | 512bp | chr12:110013662+110014173 |
4 | CCATGTTCCAATTCCAGTGAC | GAGAGCATGTGCATTCTCCAG | 346bp | chr12:110017502+110017847 |
5 | CTGGACCAGATGCTTGGAGTC | GGAGGTGGTGAGGTCTCAGTG | 345bp | chr12:110019096+110019440 |
6 | TCCGTAGCTGGAGAGGTTCAG | TCCTGAACCTACAACTCTCTTGG | 519bp | chr12:110023674+110024192 |
7 | CTGGCAAGAGCTTCCTGGTG | GGAGAAGGAGAGAGCAGGTC | 434bp | chr12:110024360+110024793 |
8 | GACTGCTCCCAGTCCTGTCC | CCCTATAACCCATCAGCCAGAG | 259bp | chr12:110028504+110028762 |
9 | GGCTGTGTGAACACCTCCTC | AGCACAGCCAGATTGCAGAG | 298bp | chr12:110028945+110029242 |
10 | TGTCTCCAGCCAACAACTGTC | TTCAAGGGAATTCTCCAGGTG | 334bp | chr12:110032727+110033060 |
11a | CTTTTGCCTTGAATATGATGAGC | AGAATAATCCAGAAAGGGGCATC | 281bp | chr12:110034155+110034435 |