MVK
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
2 TAGCAAGTGCTGGACGGTAAC TTGCTATCATACCGAGCGAG 413bp chr12:110012486+110012898
3 GCCAAGGTTGATTAGCAGTGTC GCCTTGATGATCTGTCCACAAC 512bp chr12:110013662+110014173
4 CCATGTTCCAATTCCAGTGAC GAGAGCATGTGCATTCTCCAG 346bp chr12:110017502+110017847
5 CTGGACCAGATGCTTGGAGTC GGAGGTGGTGAGGTCTCAGTG 345bp chr12:110019096+110019440
6 TCCGTAGCTGGAGAGGTTCAG TCCTGAACCTACAACTCTCTTGG 519bp chr12:110023674+110024192
7 CTGGCAAGAGCTTCCTGGTG GGAGAAGGAGAGAGCAGGTC 434bp chr12:110024360+110024793
8 GACTGCTCCCAGTCCTGTCC CCCTATAACCCATCAGCCAGAG 259bp chr12:110028504+110028762
9 GGCTGTGTGAACACCTCCTC AGCACAGCCAGATTGCAGAG 298bp chr12:110028945+110029242
10 TGTCTCCAGCCAACAACTGTC TTCAAGGGAATTCTCCAGGTG 334bp chr12:110032727+110033060
11a CTTTTGCCTTGAATATGATGAGC AGAATAATCCAGAAAGGGGCATC 281bp chr12:110034155+110034435

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
2 TAGCAAGTGCTGGACGGTAAC TTGCTATCATACCGAGCGAG 413bp chr12:110012486+110012898
3 GCCAAGGTTGATTAGCAGTGTC GCCTTGATGATCTGTCCACAAC 512bp chr12:110013662+110014173
4 CCATGTTCCAATTCCAGTGAC GAGAGCATGTGCATTCTCCAG 346bp chr12:110017502+110017847
5 CTGGACCAGATGCTTGGAGTC GGAGGTGGTGAGGTCTCAGTG 345bp chr12:110019096+110019440
6 TCCGTAGCTGGAGAGGTTCAG TCCTGAACCTACAACTCTCTTGG 519bp chr12:110023674+110024192
7 CTGGCAAGAGCTTCCTGGTG GGAGAAGGAGAGAGCAGGTC 434bp chr12:110024360+110024793
8 GACTGCTCCCAGTCCTGTCC CCCTATAACCCATCAGCCAGAG 259bp chr12:110028504+110028762
9 GGCTGTGTGAACACCTCCTC AGCACAGCCAGATTGCAGAG 298bp chr12:110028945+110029242
10 TGTCTCCAGCCAACAACTGTC TTCAAGGGAATTCTCCAGGTG 334bp chr12:110032727+110033060
11a CTTTTGCCTTGAATATGATGAGC AGAATAATCCAGAAAGGGGCATC 281bp chr12:110034155+110034435

IV. Ethanol precipitation

V. Sequencing analysis