NP
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGGTTCAGCCGATTGAATTAG ACACTAGGCTCAGTGCCAGG 394bp chr14:20937444+20937837
2 GTTCAGTGGATCCAACACTG CATGTCCTGTCTCTTATGGG 660bp chr14:20940179+20940838
3 AGTAATGCCTGGCTCTCTCAC TCCTGCTGCATTGGTGACTAC 549bp chr14:20942452+20943000
4-5 CACTACCTAGCTATCTGGGC GCTACACCAAATCTCACCAG 780bp chr14:20942799+20943578
6a GTTGAAAGCGAGGCTAAAGG AGTCCTTGTTTGGTCTCTGG 718bp chr14:20944464+20945181

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGGTTCAGCCGATTGAATTAG ACACTAGGCTCAGTGCCAGG 394bp chr14:20937444+20937837
2 GTTCAGTGGATCCAACACTG CATGTCCTGTCTCTTATGGG 660bp chr14:20940179+20940838
3 AGTAATGCCTGGCTCTCTCAC TCCTGCTGCATTGGTGACTAC 549bp chr14:20942452+20943000
4-5 CACTACCTAGCTATCTGGGC GCTACACCAAATCTCACCAG 780bp chr14:20942799+20943578
6a GTTGAAAGCGAGGCTAAAGG AGTCCTTGTTTGGTCTCTGG 718bp chr14:20944464+20945181

IV. Ethanol precipitation

V. Sequencing analysis