TNFRSF13B
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GCCCGGCAGGCCTTCCACT TGGCTTCTGTCACGGAAGGCA 484bp chr17:16875117-16875600
2 GCCCCAGGCCCCTAGCAAGTG TCTCCTCCTGCCACCCTTTCCTCA 431bp chr17:16855648-16856078
3 GGCTTACTCTGGAATTGCCTTCTG CTTCTGGCCATTTGCTTGGACT 503bp chr17:16851925-16852427
4 CCAGCCTCTCCAGGAGCCAGAC CCGGGTGCCACTCTCCCAGTTA 497bp chr17:16843449-16843945
5 ACAGGTTCTGGTCAGCAACTG TGGGAACTGGGACTCAGAGT 761bp chr17:16842519-16843279

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GAGACCCAATGCACAGGCAT TGGCTTCTGTCACGGAAGGCA 404bp chr17:16875117-16875520
2 GCCCCAGGCCCCTAGCAAGTG TCTCCTCCTGCCACCCTTTCCTCA 431bp chr17:16855648-16856078
3 GTCCTTTGTGGTCAAACCCAG CTTCTGGCCATTTGCTTGGACT 446bp chr17:16851925-16852370
4 ATGGGGGGATGTGGATTGCT CCGGGTGCCACTCTCCCAGTTA 458bp chr17:16843449-16843906
5 ACAGGTTCTGGTCAGCAACTG CTCTCTCCCTCCTCCTTTCC 454bp chr17:16842826-16843279

IV. Ethanol precipitation

V. Sequencing analysis