Forward sequence | Reverse sequence | |||
1 | CAAAGCGAATGAAAGAATAGGC | ACGTGGAGGCTATTTGGAAAG | 510bp | chr12:109535240+109535749 |
2 | CGCGAAAAGACCACGTGGGG | GGGTACAACCTAAGCCTACG | 635bp | chr12:109535919+109536553 |
3 | GGGCAGGCACTGTTTTTGTT | GCTTAACTCCAGTGTCCCTA | 337bp | chr12:109536833+109537169 |
4 | AGGGTCTGTGCTGCTTACAT | CCCAGATAGTCCCACTTGTA | 242bp | chr12:109539648+109539889 |
5 | CATATGTGCCAGTGTCCCTTC | TACCATCAACAATGGAGTCGG | 569bp | chr12:109540513+109541081 |
6 | GCAGGGAAGCACAGCTTGCT | CCCTTATAACAGGCCTAAGGGA | 454bp | chr12:109541109+109541562 |
7 | CCACTGCAGCAAGACTCTGTC | TGAGGACTCCTTCAAAGATCAAG | 721bp | chr12:109547546+109548266 |
Exon No. | Forward sequence | Reverse sequence | Expected PCR product size (bp) |
Genomic regions amplified by ePCR with the primer |
1 | GCACCGTTGCACGCCCCACCC | ACGTGGAGGCTATTTGGAAAG | chr12:?-109535749 | |
2 | CGCGAAAAGACCACGTGGGG | GGGTACAACCTAAGCCTACG | 635bp | chr12:109535919+109536553 |
3 | GAATTTGGACATAACATGACAAAGA | GCTTAACTCCAGTGTCCCTA | 302bp | chr12:109536868+109537169 |
4 | AGGGTCTGTGCTGCTTACAT | CCCAGATAGTCCCACTTGTA | 242bp | chr12:109539648+109539889 |
5 | TTTCTTGTGGCTTGCTTTCAG | AATCTAGCAGTCGCTGGCTTAC | 132bp | chr12:109540623+109540754 |
6 | GCAGGGAAGCACAGCTTGCT | CCCTTATAACAGGCCTAAGGGA | 454bp | chr12:109541109+109541562 |
7 | GTCCCAAATCTGCCACCATT | TGAGGACTCCTTCAAAGATCAAG | 672bp | chr12:109547595+109548266 |