UNG
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
1 CAAAGCGAATGAAAGAATAGGC ACGTGGAGGCTATTTGGAAAG 510bp chr12:109535240+109535749
2 CGCGAAAAGACCACGTGGGG GGGTACAACCTAAGCCTACG 635bp chr12:109535919+109536553
3 GGGCAGGCACTGTTTTTGTT GCTTAACTCCAGTGTCCCTA 337bp chr12:109536833+109537169
4 AGGGTCTGTGCTGCTTACAT CCCAGATAGTCCCACTTGTA 242bp chr12:109539648+109539889
5 CATATGTGCCAGTGTCCCTTC TACCATCAACAATGGAGTCGG 569bp chr12:109540513+109541081
6 GCAGGGAAGCACAGCTTGCT CCCTTATAACAGGCCTAAGGGA 454bp chr12:109541109+109541562
7 CCACTGCAGCAAGACTCTGTC TGAGGACTCCTTCAAAGATCAAG 721bp chr12:109547546+109548266

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
1 GCACCGTTGCACGCCCCACCC ACGTGGAGGCTATTTGGAAAG chr12:?-109535749
2 CGCGAAAAGACCACGTGGGG GGGTACAACCTAAGCCTACG 635bp chr12:109535919+109536553
3 GAATTTGGACATAACATGACAAAGA GCTTAACTCCAGTGTCCCTA 302bp chr12:109536868+109537169
4 AGGGTCTGTGCTGCTTACAT CCCAGATAGTCCCACTTGTA 242bp chr12:109539648+109539889
5 TTTCTTGTGGCTTGCTTTCAG AATCTAGCAGTCGCTGGCTTAC 132bp chr12:109540623+109540754
6 GCAGGGAAGCACAGCTTGCT CCCTTATAACAGGCCTAAGGGA 454bp chr12:109541109+109541562
7 GTCCCAAATCTGCCACCATT TGAGGACTCCTTCAAAGATCAAG 672bp chr12:109547595+109548266

IV. Ethanol precipitation

V. Sequencing analysis