VPREB1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGAAAGTCTGGGATGGTAGTG AGAAATATCTCAGCAGGAACC 442bp chr22:22599087+22599528
2 CTGCTCATGCTGTTTGTCTAC AGAATCAGATGACACCAGAGG 881bp chr22:22599244+22600124

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGAAAGTCTGGGATGGTAGTG AGAAATATCTCAGCAGGAACC 442bp chr22:22599087+22599528
2 CTGCTCATGCTGTTTGTCTAC AGAATCAGATGACACCAGAGG 881bp chr22:22599244+22600124

IV. Ethanol precipitation

V. Sequencing analysis