G6PC3
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ATATCCGCCTTCGCATCAGC GGATGGTTGGACTCTGAGGTT 816bp chr17:42147921+42148736
2 GAGCAGTGGACAGAATCGTAGTC CCAGTAATTGAGAGGCTGGTCC 357bp chr17:42151392+42151748
3-4 GCCTCAGAGCAGAGCGAGTT GGAGCGATGGGAAGATCCTA 633bp chr17:42151904+42152536
5 TCTCTTGCCAAGCTGCACTG TGAACCAGAGACACACCAGG 312bp chr17:42152587+42152898
6a GCAGATGGCCAAGAGCCAG CTCCTGGAGTGTGGTGGTCA 502bp chr17:42152979+42153480

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ATATCCGCCTTCGCATCAGC GGATGGTTGGACTCTGAGGTT 816bp chr17:42147921+42148736
2 GAGCAGTGGACAGAATCGTAGTC CCAGTAATTGAGAGGCTGGTCC 357bp chr17:42151392+42151748
3-4 GCCTCAGAGCAGAGCGAGTT GGAGCGATGGGAAGATCCTA 633bp chr17:42151904+42152536
5 TCTCTTGCCAAGCTGCACTG TGAACCAGAGACACACCAGG 312bp chr17:42152587+42152898
6a GCAGATGGCCAAGAGCCAG CTCCTGGAGTGTGGTGGTCA 502bp chr17:42152979+42153480

IV. Ethanol precipitation

V. Sequencing analysis