CARD9
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CTGAGAAGGAGTGGGAGCTG GTTGAGGTTGGGCCTCAGT 423bp chr9:139266200-139266622
3 ACCACGGCACACCTCATCT CAGGCCAGTGTCAGATGGT 425bp chr9:139265606-139266030
4 TCCATGATCATCGGTGAGTG CAGAAGGTCCAGTGAGCCTG 632bp chr9:139265157-139265788
5-6 CGCACCAGAGTGAGGAGAAG AAGGAGAGGGCACCGTCCA 765bp chr9:139264587-139265351
7 CTGGCCTGACCACATGCTT GGATTGCTTGAGGCCAGTAG 397bp chr9:139264032-139264428
8 CTGACTTCGTGTCCGCAGAG TCACAGAGAAGGGTTGGGTC 464bp chr9:139261983-139262446
9-10 GCAGCCTAGCAGGTTGTTCT CTGTGCTGAAGATGGGCCT 683bp chr9:139261183-139261865
11 GCCATAAGGCCTCAGTCTCA AGGGAGCCACTCTCCCTGT 324bp chr9:139259519-139259842
12 GCTGAGCTGAGGCTGTCTG CCTGTGAAGACAGGTGTCTCAG 290bp chr9:139258853-139259142
13 AGAGAGTTTTGAGAACTACCGCA GGTGGTGAGCACCCGCAT 423bp chr9:139258536-139258958

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 CTGAGAAGGAGTGGGAGCTG GTTGAGGTTGGGCCTCAGT 423bp chr9:139266200-139266622
3 ACCACGGCACACCTCATCT CAGGCCAGTGTCAGATGGT 425bp chr9:139265606-139266030
4 TCCATGATCATCGGTGAGTG CAGAAGGTCCAGTGAGCCTG 632bp chr9:139265157-139265788
5-6 CGCACCAGAGTGAGGAGAAG CCTCCAGGAGTGGGTGAGT 741bp chr9:139264611-139265351
7 CTGGCCTGACCACATGCTT GGATTGCTTGAGGCCAGTAG 397bp chr9:139264032-139264428
8 CTGACTTCGTGTCCGCAGAG TCACAGAGAAGGGTTGGGTC 464bp chr9:139261983-139262446
9-10 GCAGCCTAGCAGGTTGTTCT CTGTGCTGAAGATGGGCCT 683bp chr9:139261183-139261865
11 GCCATAAGGCCTCAGTCTCA AGGGAGCCACTCTCCCTGT 324bp chr9:139259519-139259842
12 GCTGAGCTGAGGCTGTCTG CCTGTGAAGACAGGTGTCTCAG 290bp chr9:139258853-139259142
13 AGAGAGTTTTGAGAACTACCGCA GGTGGTGAGCACCCGCAT 423bp chr9:139258536-139258958

IV. Ethanol precipitation

V. Sequencing analysis