AICDA
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TAACCTGTGTGACTCTGCCTAT AGGGATAGCTACCAGTCTCAT 339bp chr12:8765273-8765611
2 CTGAAGGAAAATCCAGAGTGACC ATCAGCAGGTGGCTCTAAAAGC 291bp chr12:8759390-8759680
3 AATGACTAAGGCTACCAGAGCCG CCTTGTCTCTGAGCCATTCACT 519bp chr12:8757632-8758150
4 GATTCTGGTTCACCTCTGGAG AGGAGAAATAAAGTTTTCATCAG 433bp chr12:8757272-8757704
5 CTCAGTTTAGAAGACTCTCCGGC CCACTGTCTTCAGCAGAGATATTTC 194bp chr12:8756826-8757019

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TAACCTGTGTGACTCTGCCTAT AGGGATAGCTACCAGTCTCAT 339bp chr12:8765273-8765611
2 CTGAAGGAAAATCCAGAGTGACC ATCAGCAGGTGGCTCTAAAAGC 291bp chr12:8759390-8759680
3 AATGACTAAGGCTACCAGAGCCG CCTTGTCTCTGAGCCATTCACT 519bp chr12:8757632-8758150
4 GATTCTGGTTCACCTCTGGAG AGGAGAAATAAAGTTTTCATCAG 433bp chr12:8757272-8757704
5 CTCAGTTTAGAAGACTCTCCGGC CCACTGTCTTCAGCAGAGATATTTC 194bp chr12:8756826-8757019

IV. Ethanol precipitation

V. Sequencing analysis